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验证数据展示

  • WB analysis of HEK-293 using 67072-1-Ig (same clone as 67072-1-PBS)

    WB analysis of HEK-293 using 67072-1-Ig (same clone as 67072-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 67072-1-Ig (same clone as 67072-1-PBS)

    WB analysis of HSC-T6 using 67072-1-Ig (same clone as 67072-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 67072-1-Ig (same clone as 67072-1-PBS)

    IHC staining of human pancreas cancer using 67072-1-Ig (same clone as 67072-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

  • IF Staining of RAW 264.7 using 67072-1-Ig (same clone as 67072-1-PBS)

    IF Staining of RAW 264.7 using 67072-1-Ig (same clone as 67072-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using ASK1 antibody (67072-1-Ig, Clone: 1C3A10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

ASK1 Monoclonal antibody, PBS Only

ASK1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67072-1-PBS

产品说明书

CloneNo. 1C3A10

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ASK 1, ASK1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67072-1-PBS targets ASK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原

CatNo: Ag5818

Product name: Recombinant human ASK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 681-936 aa of BC054503

Sequence: GDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRACANDLLVDE

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 mitogen-activated protein kinase kinase kinase 5
别名 ASK 1, ASK1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5
计算分子量 155 kDa
观测分子量 110 kDa
GenBank蛋白编号BC054503
基因名称 ASK1
Gene ID (NCBI) 4217
RRIDAB_2882381
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ99683
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. It is an evolutionarily conserved mitogen activated protein 3-kinase that activates both Jnk and p38 mitogen-activated protein kinases. ASK1 is activated in response to various cytotoxic stresses including TNF, Fas and reactive oxygen species (ROS) such as H2O2, and activates c-Jun NH 2-terminal kinase (JNK) and p38. 67072-1-Ig antibody detects the degraded product of ASK1 in SDS-PAGE.

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