• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67859-1-Ig

    WB analysis using 67859-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67859-1-Ig (ASH2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67859-1-Ig

    WB analysis using 67859-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67859-1-Ig (ASH2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67859-1-Ig

    IHC staining of human colon cancer using 67859-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67859-1-Ig (ASH2L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ASH2L antibody included
  • FC experiment of HeLa using 67859-1-Ig

    FC experiment of HeLa using 67859-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ASH2L (67859-1-Ig, Clone:1G1H3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

ASH2L Monoclonal antibody

ASH2L Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 67859-1-Ig

产品说明书

CloneNo. 1G1H3

宿主/亚型

Mouse / IgG2a

种属反应性

human, rat

应用

WB, IHC, FC (Intra), ELISA

别名

Bre2, ASH2-like protein, ASH2L2, ASH2L1, ASH2 like protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inLNCaP cells, HSC-T6 cells, HeLa cells, HEK-293 cells, PC-12 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ASH2L Ready-To-Use IHC Kit. ASH2L primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67859-1-Ig targets ASH2L in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, rat
免疫原 ASH2L fusion protein Ag3008 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ash2 (absent, small, or homeotic)-like (Drosophila)
别名 Bre2, ASH2-like protein, ASH2L2, ASH2L1, ASH2 like protein
计算分子量 628 aa, 69 kDa
观测分子量 80 kDa
GenBank蛋白编号BC015936
基因名称 ASH2L
Gene ID (NCBI) 9070
RRIDAB_2918617
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UBL3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ASH2L antibody 67859-1-IgDownload protocol
IHC protocol for ASH2L antibody 67859-1-IgDownload protocol
FC protocol for ASH2L antibody 67859-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols