验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, HSC-T6 cells, HeLa cells, HEK-293 cells, PC-12 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy ASH2L Ready-To-Use IHC Kit. ASH2L primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67859-1-Ig targets ASH2L in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, FC (Intra), ELISA Application Description |
| 经测试反应性 | human, rat |
| 免疫原 |
CatNo: Ag3008 Product name: Recombinant human ASH2L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 234-534 aa of BC015936 Sequence: HGYPLEHPFNKDGYRYILAEPDPHAPDPEKLELDCWAGKPIPGDLYRACLYERVLLALHDRAPQLKISDDRLTVVGEKGYSMVRASHGVRKGAWYFEITVDEMPPDTAARLGWSQPLGNLQAPLGYDKFSYSWRSKKGTKFHQSIGKHYSSGYGQGDVLGFYINLPEDTETAKSLPDTYKDKALIKFKSYLYFEEKDFVDKAEKSLKQTPHSEIIFYKNGVNQGVAYKDIFEGVYFPAISLYKSCTVSINFGPCFKYPPKDLTYRPMSDMGWGAVVEHTLADVLYHVETEVDGRRSPPWEP 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ash2 (absent, small, or homeotic)-like (Drosophila) |
| 别名 | Bre2, ASH2-like protein, ASH2L2, ASH2L1, ASH2 like protein |
| 计算分子量 | 628 aa, 69 kDa |
| 观测分子量 | 80 kDa |
| GenBank蛋白编号 | BC015936 |
| 基因名称 | ASH2L |
| Gene ID (NCBI) | 9070 |
| RRID | AB_2918617 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UBL3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for ASH2L antibody 67859-1-Ig | Download protocol |
| IHC protocol for ASH2L antibody 67859-1-Ig | Download protocol |
| WB protocol for ASH2L antibody 67859-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
