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验证数据展示

  • WB analysis using 11529-1-AP (same clone as 11529-1-PBS)

    WB analysis using 11529-1-AP (same clone as 11529-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11529-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 11529-1-AP (same clone as 11529-1-PBS)

    IP experiment of HeLa using 11529-1-AP (same clone as 11529-1-PBS)

    IP result of anti-ASCC2 (IP:11529-1-AP, 4ug; Detection:11529-1-AP 1:1000) with HeLa cells lysate 2640ug. This data was developed using the same antibody clone with 11529-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 11529-1-AP (same clone as 11529-1-PBS)

    IF Staining of HepG2 using 11529-1-AP (same clone as 11529-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ASCC2 antibody (11529-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 11529-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 11529-1-AP (same clone as 11529-1-PBS)

    FC experiment of HepG2 using 11529-1-AP (same clone as 11529-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug ASCC2 Polyclonal antibody (11529-1-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 11529-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 11529-1-AP (same clone as 11529-1-PBS)

    IHC staining of human colon cancer using 11529-1-AP (same clone as 11529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11529-1-AP (ASCC2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11529-1-PBS in a different storage buffer formulation.

ASCC2 Polyclonal antibody, PBS Only

ASCC2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 11529-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

ASC-1 complex subunit p100, ASC1P100, RQT3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

11529-1-PBS targets ASCC2 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2100

Product name: Recombinant human ASCC2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 408-757 aa of BC025368

Sequence: RRKATDAKDPSVIEEPNGEPNGVTVTAEAVSQASSHPENSEEEECMGAAAAVGPAMCGVELDSLISQVKDLLPDLGEGFILACLEYYHYDPEQVINNILEERLAPTLSQLDRNLDREMKPDPTPLLTSRHNVFQNDEFDVFSRDSVDLSRVHKGKSTRKEENTRSLLNDKRAVAAQRQRYEQYSVVVEEVPLQPGESLPYHSVYYEDEYDDTYDGNQVGANDADSDDELISRRPFTIPQVLRTKVPREGQEEDDDDEEDDADEEAPKPDHFVQDPAVLREKAEARRMAFLAKKGYRHDSSTAVAGSPRGHGQSRETTQERRKKEANKATRANHNRRTMADRKRSKGMIPS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 activating signal cointegrator 1 complex subunit 2
别名 ASC-1 complex subunit p100, ASC1P100, RQT3
计算分子量 757 aa, 86 kDa
观测分子量 90-100 kDa
GenBank蛋白编号BC025368
基因名称 ASCC2
Gene ID (NCBI) 84164
RRIDAB_2059358
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H1I8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ASCC2, also known as ASC1p100, is a subunit of the activating signal cointegrator 1 complex. ASCC2 plays a role in DNA damage repair as a component of the ASCC complex, recruiting ASCC3 and ALKBH3 to sites of DNA damage by binding to polyubiquitinated proteins that have 'Lys-63'-linked polyubiquitin chains. Additionally, it is part of the ASC-1 complex that enhances NF-kappa-B, SRF, and AP1 transactivation(PMID: 29144457, 12077347).

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