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验证数据展示

  • WB analysis using 67494-1-Ig (same clone as 67494-1-PBS)

    WB analysis using 67494-1-Ig (same clone as 67494-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67494-1-Ig (ASC/TMS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

  • WB analysis of human placenta using 67494-1-Ig (same clone as 67494-1-PBS)

    WB analysis of human placenta using 67494-1-Ig (same clone as 67494-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67494-1-Ig (ASC/TMS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67494-1-Ig (same clone as 67494-1-PBS)

    IHC staining of human colon cancer using 67494-1-Ig (same clone as 67494-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67494-1-Ig (ASC/TMS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ASC/TMS1 antibody included
  • IF Staining of HeLa using 67494-1-Ig (same clone as 67494-1-PBS)

    IF Staining of HeLa using 67494-1-Ig (same clone as 67494-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ASC/TMS1 antibody (67494-1-Ig, Clone: 2G12E6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 67494-1-Ig (same clone as 67494-1-PBS)

    IF Staining of MCF-7 using 67494-1-Ig (same clone as 67494-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ASC/TMS1 antibody (67494-1-Ig, Clone: 2G12E6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 67494-1-Ig (same clone as 67494-1-PBS)

    IF Staining of THP-1 using 67494-1-Ig (same clone as 67494-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using ASC/TMS1 antibody (67494-1-Ig, Clone: 2G12E6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

ASC/TMS1 Monoclonal antibody, PBS Only

ASC/TMS1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67494-1-PBS

产品说明书

CloneNo. 2G12E6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

PYCARD, 2G12E6, Apoptosis-associated speck-like protein containing a CARD, ASC, CARD5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ASC/TMS1 Ready-To-Use IHC Kit. ASC/TMS1 primary antibody included.

产品信息

67494-1-PBS targets ASC/TMS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag28424

Product name: Recombinant human TMS1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-135 aa of BC004470

Sequence: MGRARDAILDALENLTAEELKKFKLQAATHQGSGAAPAGIQAPPQSAAKPGLHFIDQHRAALIARVTNVEWLLDALYGKVLTDEQYQAVRAEPTNPSKMRKLFSFTPAWNWTCKDLLLQALRESQSYLVEDLERS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 PYD and CARD domain containing
别名 PYCARD, 2G12E6, Apoptosis-associated speck-like protein containing a CARD, ASC, CARD5
计算分子量 25 kDa
观测分子量 22-25 kDa
GenBank蛋白编号BC004470
基因名称 ASC/TMS1
Gene ID (NCBI) 29108
RRIDAB_2882718
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9ULZ3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.

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