验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, human placenta tissue, THP-1 cells, U-937 cells | 
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 | 
| Positive IF/ICC detected in | HeLa cells, THP-1 cells, MCF-7 cells | 
推荐稀释比
| 应用 | 推荐稀释比 | 
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 | 
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 | 
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67494-1-Ig targets ASC/TMS1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description | 
| 文献引用应用 | WB, IHC, IF, IP, CoIP | 
| 经测试反应性 | human | 
| 文献引用反应性 | human | 
| 免疫原 | CatNo: Ag28424 Product name: Recombinant human TMS1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-135 aa of BC004470 Sequence: MGRARDAILDALENLTAEELKKFKLQAATHQGSGAAPAGIQAPPQSAAKPGLHFIDQHRAALIARVTNVEWLLDALYGKVLTDEQYQAVRAEPTNPSKMRKLFSFTPAWNWTCKDLLLQALRESQSYLVEDLERS种属同源性预测 | 
| 宿主/亚型 | Mouse / IgG1 | 
| 抗体类别 | Monoclonal | 
| 产品类型 | Antibody | 
| 全称 | PYD and CARD domain containing | 
| 别名 | PYCARD, 2G12E6, Apoptosis-associated speck-like protein containing a CARD, ASC, CARD5 | 
| 计算分子量 | 25 kDa | 
| 观测分子量 | 22-25 kDa | 
| GenBank蛋白编号 | BC004470 | 
| 基因名称 | ASC/TMS1 | 
| Gene ID (NCBI) | 29108 | 
| RRID | AB_2882718 | 
| 偶联类型 | Unconjugated | 
| 形式 | Liquid | 
| 纯化方式 | Protein G purification | 
| UNIPROT ID | Q9ULZ3 | 
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. | 
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. | 
背景介绍
PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ASC/TMS1 antibody 67494-1-Ig | Download protocol | 
| WB protocol for ASC/TMS1 antibody 67494-1-Ig | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 
发表文章
| Species | Application | Title | 
|---|---|---|
| Mol Cell An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation | ||
| Mol Cell Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation | ||
| Sci Adv Asparagine transporter supports macrophage inflammation via histone phosphorylation | ||
| Oxid Med Cell Longev PPARγ Alleviates Sepsis-Induced Liver Injury by Inhibiting Hepatocyte Pyroptosis via Inhibition of the ROS/TXNIP/NLRP3 Signaling Pathway. | ||
| J Neuroinflammation Upregulation of TRPC5 in hippocampal excitatory synapses improves memory impairment associated with neuroinflammation in microglia knockout IL-10 mice. | ||
| J Virol Pseudorabies Virus Infection Activates the TLR-NF-κB Axis and AIM2 Inflammasome To Enhance Inflammatory Responses in Mice | 







