ASC/TMS1 Monoclonal antibody

ASC/TMS1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67494-1-Ig

产品说明书

CloneNo. 2G12E6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA and More (3)

PYCARD, 2G12E6, Apoptosis-associated speck-like protein containing a CARD, ASC, CARD5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHL-60 cells, human placenta tissue, THP-1 cells, U-937 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, THP-1 cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy ASC/TMS1 Ready-To-Use IHC Kit. ASC/TMS1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67494-1-Ig targets ASC/TMS1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag28424

Product name: Recombinant human TMS1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-135 aa of BC004470

Sequence: MGRARDAILDALENLTAEELKKFKLQAATHQGSGAAPAGIQAPPQSAAKPGLHFIDQHRAALIARVTNVEWLLDALYGKVLTDEQYQAVRAEPTNPSKMRKLFSFTPAWNWTCKDLLLQALRESQSYLVEDLERS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 PYD and CARD domain containing
别名 PYCARD, 2G12E6, Apoptosis-associated speck-like protein containing a CARD, ASC, CARD5
计算分子量 25 kDa
观测分子量 22-25 kDa
GenBank蛋白编号BC004470
基因名称 ASC/TMS1
Gene ID (NCBI) 29108
RRIDAB_2882718
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9ULZ3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.

实验方案

Product Specific Protocols
WB protocol for ASC/TMS1 antibody 67494-1-IgDownload protocol
IHC protocol for ASC/TMS1 antibody 67494-1-IgDownload protocol
IF protocol for ASC/TMS1 antibody 67494-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

Mol Cell

An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation

Authors - Stephanie Pei Tung Yiu
WB,IP,IF

Mol Cell

Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation

Authors - Chuanlong Wang
WB,IF

Sci Adv

Asparagine transporter supports macrophage inflammation via histone phosphorylation

Authors - Chuanlong Wang
WB

Oxid Med Cell Longev

PPARγ Alleviates Sepsis-Induced Liver Injury by Inhibiting Hepatocyte Pyroptosis via Inhibition of the ROS/TXNIP/NLRP3 Signaling Pathway.

Authors - Zeyu Li
WB

J Neuroinflammation

Upregulation of TRPC5 in hippocampal excitatory synapses improves memory impairment associated with neuroinflammation in microglia knockout IL-10 mice.

Authors - Shiji Huo
WB,IF

J Virol

Pseudorabies Virus Infection Activates the TLR-NF-κB Axis and AIM2 Inflammasome To Enhance Inflammatory Responses in Mice

Authors - Qiongqiong Zhou
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