ASC/TMS1 Monoclonal antibody

ASC/TMS1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67494-1-Ig

产品说明书

CloneNo. 2G12E6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, ELISA and More (3)

PYCARD, 2G12E6, Apoptosis-associated speck-like protein containing a CARD, ASC, CARD5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHL-60 cells, human placenta tissue, THP-1 cells, U-937 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ASC/TMS1 Ready-To-Use IHC Kit. ASC/TMS1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67494-1-Ig targets ASC/TMS1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human
文献引用反应性human
免疫原 ASC/TMS1 fusion protein Ag28424 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 PYD and CARD domain containing
别名 PYCARD, 2G12E6, Apoptosis-associated speck-like protein containing a CARD, ASC, CARD5
计算分子量 25 kDa
观测分子量 22-25 kDa
GenBank蛋白编号BC004470
基因名称 ASC/TMS1
Gene ID (NCBI) 29108
RRIDAB_2882718
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9ULZ3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.

实验方案

Product Specific Protocols
WB protocol for ASC/TMS1 antibody 67494-1-IgDownload protocol
IHC protocol for ASC/TMS1 antibody 67494-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

Mol Cell

An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation

Authors - Stephanie Pei Tung Yiu
WB,IP,IF

Mol Cell

Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation

Authors - Chuanlong Wang
WB,IF

Sci Adv

Asparagine transporter supports macrophage inflammation via histone phosphorylation

Authors - Chuanlong Wang
WB

Oxid Med Cell Longev

PPARγ Alleviates Sepsis-Induced Liver Injury by Inhibiting Hepatocyte Pyroptosis via Inhibition of the ROS/TXNIP/NLRP3 Signaling Pathway.

Authors - Zeyu Li
WB

J Neuroinflammation

Upregulation of TRPC5 in hippocampal excitatory synapses improves memory impairment associated with neuroinflammation in microglia knockout IL-10 mice.

Authors - Shiji Huo
WB,IF

J Virol

Pseudorabies Virus Infection Activates the TLR-NF-κB Axis and AIM2 Inflammasome To Enhance Inflammatory Responses in Mice

Authors - Qiongqiong Zhou
Loading...