• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ASB13 Polyclonal antibody

ASB13 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 25616-1-AP

Print datasheet

Synonyms

ASB 13, ASB13

验证数据展示

  • WB analysis using 25616-1-AP

    WB analysis using 25616-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25616-1-AP (ASB13 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 25616-1-AP

    IHC staining of human small intestine using 25616-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25616-1-AP (ASB13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 25616-1-AP

    IF Staining of HepG2 using 25616-1-AP

    Immunofluorescent analysis of HepG2 cells using 25616-1-AP (ASB13 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inL02 cells, mouse heart tissue, mouse liver tissue, rat heart tissue, rat liver tissue
Positive IHC detected inhuman small intestine tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25616-1-AP is ASB13 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen ASB13 fusion protein Ag22533 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ankyrin repeat and SOCS box-containing 13
Synonyms ASB 13, ASB13
Calculated Molecular Weight 278 aa, 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC012056
Gene Symbol ASB13
Gene ID (NCBI) 79754
RRIDAB_2880162
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WXK3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ASB13 antibody 25616-1-APDownload protocol
IHC protocol for ASB13 antibody 25616-1-APDownload protocol
IF protocol for ASB13 antibody 25616-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols