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验证数据展示

  • WB analysis using 25616-1-AP

    WB analysis using 25616-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25616-1-AP (ASB13 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 25616-1-AP

    IHC staining of human small intestine using 25616-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25616-1-AP (ASB13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 25616-1-AP

    IF Staining of HepG2 using 25616-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ASB13 antibody (25616-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

ASB13 Polyclonal antibody

ASB13 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25616-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

ASB-13, ASB 13

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
Biotin
规格价格库存

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经过测试的应用

Positive WB detected inL02 cells, mouse heart tissue, mouse liver tissue, rat heart tissue, rat liver tissue
Positive IHC detected inhuman small intestine tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25616-1-AP targets ASB13 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag22533

Product name: Recombinant human ASB13 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 5-173 aa of BC012056

Sequence: AADGCFLGDVGFWVERTPVHEAAQRGESLQLQQLIESGACVNQVTVDSITPLHAASLQGQARCVQLLLAAGAQVDARNIDGSTPLCDACASGSIECVKLLLSYGAKVNPPLYTASPLHEACMSGSSECVRLLIDVGANLEAHDCHFGTPLHVACAREHLDCVKVLLNAA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ankyrin repeat and SOCS box-containing 13
别名 ASB-13, ASB 13
计算分子量 278 aa, 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC012056
基因名称 ASB13
Gene ID (NCBI) 79754
RRIDAB_2880162
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WXK3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ASB13 antibody 25616-1-APDownload protocol
IHC protocol for ASB13 antibody 25616-1-APDownload protocol
IF protocol for ASB13 antibody 25616-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols