验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue, mouse thymus tissue |
| Positive IHC detected in | mouse kidney tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Neuro-2a cells |
Planning an IHC experiment? We recommend our IHCeasy ARPC5 Ready-To-Use IHC Kit. ARPC5 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IHC | See 1 publications below |
产品信息
16717-1-AP targets ARPC5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag10144 Product name: Recombinant human ARPC5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-154 aa of BC057237 Sequence: MSKNTVSSARFRKVDVDEYDENKFVDEEDGGDGQAGPDEGEVDSCLRHSITGNMTAALQAALKNPPINTKSQAVKDRAGSIVLKVLISFKANDIEKAVQSLDKNGVDLLMKYIYKGFESPSDNSSAMLLQWHEKALAAGGVGSIVRVLTARKTV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | actin related protein 2/3 complex, subunit 5, 16kDa |
| 别名 | p16-ARC, p16 Arc, Arp2/3 complex 16 kDa subunit, ARC16, Actin-related protein 2/3 complex subunit 5 |
| 计算分子量 | 154 aa, 17 kDa |
| 观测分子量 | 18-20 kDa |
| GenBank蛋白编号 | BC057237 |
| 基因名称 | ARPC5 |
| Gene ID (NCBI) | 10092 |
| RRID | AB_1850902 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15511 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ARPC5 antibody 16717-1-AP | Download protocol |
| IHC protocol for ARPC5 antibody 16717-1-AP | Download protocol |
| WB protocol for ARPC5 antibody 16717-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Revealing the Therapeutic Targets and Mechanism of Ginsenoside Rg1 for Liver Damage Related to Anti-Oxidative Stress Using Proteomic Analysis | ||
J Neurosci The Proteome of BLOC-1 Genetic Defects Identifies the Arp2/3 Actin Polymerization Complex to Function Downstream of the Schizophrenia Susceptibility Factor Dysbindin at the Synapse. | ||
Pigment Cell Melanoma Res YAP facilitates melanoma migration through regulation of actin-related protein 2/3 complex subunit 5 (ARPC5) | ||
J Reprod Dev MiR-145 regulates steroidogenesis in mouse primary granulosa cells by targeting Arpc5 and subsequent cytoskeleton remodeling | ||
BMC Cancer ARPC5 acts as a potential prognostic biomarker that is associated with cell proliferation, migration and immune infiltrate in gliomas | ||
Heliyon Dapagliflozin impedes endothelial cell senescence by activating the SIRT1 signaling pathway in type 2 diabetes |
