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验证数据展示

  • WB analysis using 67320-1-Ig

    WB analysis using 67320-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67320-1-Ig (ARPC1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human appendicitis using 67320-1-Ig

    IHC staining of human appendicitis using 67320-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67320-1-Ig (ARPC1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARPC1B antibody included
  • IHC staining of mouse spleen using 67320-1-Ig

    IHC staining of mouse spleen using 67320-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 67320-1-Ig (ARPC1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARPC1B antibody included

ARPC1B Monoclonal antibody

ARPC1B Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67320-1-Ig

产品说明书

CloneNo. 2C8A1

宿主/亚型

Mouse / IgG1

种属反应性

Human, rat, mouse

应用

WB, IHC, ELISA

别名

ARC41, Arp2/3 complex 41 kDa subunit, ARPC1B, p40 ARC, p41 ARC

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, THP-1 cells, rat spleen tissues
Positive IHC detected inhuman appendicitis tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ARPC1B Ready-To-Use IHC Kit. ARPC1B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67320-1-Ig targets ARPC1B in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, rat, mouse
免疫原

CatNo: Ag29095

Product name: Recombinant human ARPC1B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 265-372 aa of BC007555

Sequence: CFPVLFTYDAAAGMLSFGGRLDVPKQSSQRGLTARERFQNLDKKASSEGGTAAGAGLDSLHKNSVSQISVLSGGKAKCSQFCTTGMDGGMSIWDVKSLESALKDLKIK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 actin related protein 2/3 complex, subunit 1B, 41kDa
别名 ARC41, Arp2/3 complex 41 kDa subunit, ARPC1B, p40 ARC, p41 ARC
计算分子量 41 kDa
观测分子量 38 kDa
GenBank蛋白编号BC007555
基因名称 ARPC1B
Gene ID (NCBI) 10095
RRIDAB_2882580
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO15143
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARPC1B also known as ARC41, p41-ARC, or p40-ARC, is one of seven subunits of the human Arp2/3 protein complex, that belongs to the SOP2 family. ARPC1B localizes on centrosomes and has a distinct role in centrosomal homeostasis. ARPC1B is both a physiological activator and substrate of Aurora A kinase, and these interactions help to maintain mitotic integrity in mammalian cells. In recent resaeach, ARPC1B is selected as a candidate prediction marker for sensitivity of Choroidal malignant melanomas to radiotherapy.

实验方案

Product Specific Protocols
WB protocol for ARPC1B antibody 67320-1-IgDownload protocol
IHC protocol for ARPC1B antibody 67320-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols