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验证数据展示

  • WB analysis using 13822-1-AP (same clone as 13822-1-PBS)

    WB analysis using 13822-1-AP (same clone as 13822-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13822-1-AP (ARP3/ARP3B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13822-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse spleen using 13822-1-AP (same clone as 13822-1-PBS)

    IP experiment of mouse spleen using 13822-1-AP (same clone as 13822-1-PBS)

    IP result of anti-ARP3/ARP3B (IP:13822-1-AP, 4ug; Detection:13822-1-AP 1:1000) with mouse spleen tissue lysate 4000ug. This data was developed using the same antibody clone with 13822-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 13822-1-AP (same clone as 13822-1-PBS)

    IHC staining of human kidney using 13822-1-AP (same clone as 13822-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13822-1-AP (ARP3/ARP3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13822-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARP3 antibody included
  • Featured Product
  • KD/KO Validated

ARP3/ARP3B Polyclonal antibody, PBS Only

ARP3/ARP3B Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 13822-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ARP3 Ready-To-Use IHC Kit. ARP3 primary antibody included.

产品信息

13822-1-PBS targets ARP3/ARP3B in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4922

Product name: Recombinant human ACTR3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 156-418 aa of BC044590

Sequence: RQVGERTLTGTVIDSGDGVTHVIPVAEGYVIGSCIKHIPIAGRDITYFIQQLLRDREVGIPPEQSLETAKAVKERYSYVCPDLVKEFNKYDTDGSKWIKQYTGINAISKKEFSIDVGYERFLGPEIFFHPEFANPDFTQPISEVVDEVIQNCPIDVRRPLYKNIVLSGGSTMFRDFGRRLQRDLKRTVDARLKLSEELSGGRLKPKPIDVQVITHHMQRYAVWFGGSMLASTPEFYQVCHTKKDYEEIGPSICRHNPVFGVMS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ARP3 actin-related protein 3 homolog (yeast)
别名
计算分子量 418 aa 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC044590
基因名称 ARP3/ACTR3
Gene ID (NCBI) 10096
RRIDAB_2221967
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP61158
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ARP3 (also known as ACTR3) is a major constituent of the ARP2/3 complex that is necessary for nucleating actin polymerization at filament branches. ARP3 localizes to the lamellipodia of stationary and locomoting fibroblasts. Overexpression of ARP3 has been reported in several types of cancer cells. This antibody recognizes endogenous ARP3 and may cross-react with ATCR3B.

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