验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, mouse spleen tissue, human liver tissue, A549 cells, HL-60 cells, HeLa cells, Jurkat cells, K-562 cells, PC-3 cells |
| Positive IP detected in | mouse spleen tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ARP3 Ready-To-Use IHC Kit. ARP3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13822-1-AP targets ARP3/ARP3B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | ARP3/ARP3B fusion protein Ag4922 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ARP3 actin-related protein 3 homolog (yeast) |
| 别名 | Actin like protein 3, Actin related protein 3, ACTR3, ACTR3B, Arp11, ARP3, ARP3/ARP3B, Arp4 |
| 计算分子量 | 418 aa 47 kDa |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | BC044590 |
| 基因名称 | ARP3/ACTR3 |
| Gene ID (NCBI) | 10096 |
| RRID | AB_2221967 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P61158 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARP3 (also known as ACTR3) is a major constituent of the ARP2/3 complex that is necessary for nucleating actin polymerization at filament branches. ARP3 localizes to the lamellipodia of stationary and locomoting fibroblasts. Overexpression of ARP3 has been reported in several types of cancer cells. This antibody recognizes endogenous ARP3 and may cross-react with ATCR3B.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ARP3/ARP3B antibody 13822-1-AP | Download protocol |
| IHC protocol for ARP3/ARP3B antibody 13822-1-AP | Download protocol |
| IP protocol for ARP3/ARP3B antibody 13822-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
ACS Nano Oxidation of Reduced Graphene Oxide via Cellular Redox Signaling Modulates Actin-Mediated Neurotransmission. | ||
EMBO Rep WDR63 inhibits Arp2/3-dependent actin polymerization and mediates the function of p53 in suppressing metastasis. | ||
Elife Syncytin-mediated open-ended membrane tubular connections facilitate the intercellular transfer of cargos including Cas9 protein | ||
Int J Biol Sci Rictor promotes cell migration and actin polymerization through regulating ABLIM1 phosphorylation in Hepatocellular Carcinoma. |
