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验证数据展示

  • WB analysis using 86113-1-RR (same clone as 86113-1-PBS)

    WB analysis using 86113-1-RR (same clone as 86113-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86113-1-RR (ARNTL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86113-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 86113-1-RR (same clone as 86113-1-PBS)

    WB analysis of HeLa using 86113-1-RR (same clone as 86113-1-PBS)

    WB result of ARNTL antibody (86113-1-RR; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARNTL transfected HeLa cells. This data was developed using the same antibody clone with 86113-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 86113-1-RR (same clone as 86113-1-PBS)

    IHC staining of mouse liver using 86113-1-RR (same clone as 86113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86113-1-RR (ARNTL antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86113-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BMAL1/ARNTL antibody included
  • IHC staining of rat liver using 86113-1-RR (same clone as 86113-1-PBS)

    IHC staining of rat liver using 86113-1-RR (same clone as 86113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 86113-1-RR (ARNTL antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86113-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BMAL1/ARNTL antibody included
  • IF Staining of HeLa using 86113-1-RR (same clone as 86113-1-PBS)

    IF Staining of HeLa using 86113-1-RR (same clone as 86113-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARNTL antibody (86113-1-RR, Clone: 250536E10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86113-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ARNTL Recombinant monoclonal antibody, PBS Only

ARNTL Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86113-1-PBS

产品说明书

CloneNo. 250536E10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

BMAL1, ARNT3, Aryl hydrocarbon receptor nuclear translocator-like protein 1, Basic helix-loop-helix ARNT-like protein 1, Basic-helix-loop-helix-PAS protein MOP3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy BMAL1/ARNTL Ready-To-Use IHC Kit. BMAL1/ARNTL primary antibody included.

产品信息

86113-1-PBS targets ARNTL in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5586

Product name: Recombinant human ARNTL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-303 aa of BC041129

Sequence: MADQRMDISSTISDFMSPGPTDLLSSSLGTSGVDCNRKRKGSSTDYQESMDTDKDDPHGRLEYTEHQGRIKNAREAHSQIEKRRRDKMNSFIDELASLVPTCNAMSRKLDKLTVLRMAVQHMKTLRGATNPYTEANYKPTFLSDDELKHLILRAADGFLFVVGCDRGKILFVSESVFKILNYSQNDLIGQSLFDYLHPKDIAKVKEQLSSSDTAPRERLIDAKTGLPVKTDITPGPSRLCSGARRSFFCRMKCNRPSVKVEDKDFPSTCSKKKDRKSFCTIHSTGYLKSWPPTKMGLDEDNEP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 aryl hydrocarbon receptor nuclear translocator-like
别名 BMAL1, ARNT3, Aryl hydrocarbon receptor nuclear translocator-like protein 1, Basic helix-loop-helix ARNT-like protein 1, Basic-helix-loop-helix-PAS protein MOP3
计算分子量 69 kDa
观测分子量 69-75 kDa
GenBank蛋白编号BC041129
基因名称 ARNTL
Gene ID (NCBI) 406
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO00327
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BMAL1, a basic helix-loop-helices with Per-Arnt-Sim (PAS) domain transcription factor, is one of components that has core clock function in mammals. It form a heterodimer with CLOCK, and induce the transcription of target genes by binding to E-box elements within their promoter regions. And this transcription is inhibited in a feedback loop by PER, and also by CRY proteins.Also BMAL1 is a key player in the regulation of metabolism, playing a role in adipogenesis and in the control of glucose and triglycerides levels. Due to phosphorylation modification, BMAL1 can be detected as a band in the range of 70-80 kda (PMID: 14750952).

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