验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy ARNT Ready-To-Use IHC Kit. ARNT primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84650-4-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-84650-4 targets ARNT/HIF-1 beta in applications and shows reactivity with human, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, rat |
| 免疫原 | ARNT/HIF-1 beta fusion protein Ag5507 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | aryl hydrocarbon receptor nuclear translocator |
| 别名 | ARNT, HIF-1 beta, HIF1B, 242111G2, bHLHe2 |
| 计算分子量 | 416 aa, 46 kDa, 87 kDa |
| 观测分子量 | 87 kDa |
| GenBank蛋白编号 | BC060838 |
| 基因名称 | ARNT/HIF-1 beta |
| Gene ID (NCBI) | 405 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P27540 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HIF1B(HIF-1 beta), also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ARNT/HIF-1 beta antibody CL488-84650-4 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |