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验证数据展示

  • IF Staining of HepG2 using CL594-66732

    IF Staining of HepG2 using CL594-66732

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594-conjugated ARNT,HIF1B antibody (CL594-66732, Clone: 3B12C5 ) at dilution of 1:200, CL488-Phalloidin (green).

  • FC experiment of HeLa using CL594-66732

    FC experiment of HeLa using CL594-66732

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human ARNT,HIF1B (CL594-66732, Clone:3B12C5) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite®594-conjugated ARNT,HIF1B Monoclonal antibody

ARNT,HIF1B Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66732

产品说明书

CloneNo. 3B12C5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

ARNT/HIF-1 beta, ARNT, HIF-1 beta, HIF1B, HIF 1 beta

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ARNT Ready-To-Use IHC Kit. ARNT primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66732-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66732 targets ARNT,HIF1B in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Ag5507

Product name: Recombinant human ARNT,HIF1B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-348 aa of BC060838

Sequence: MAATTANPEMTSDVPSLGPAIASGNSGPGIQGGGAIVQRAIKRRPGLDFDDDGEGNSKFLRCDDDQMSNDKERFARSDDEQSSADKERLARENHSEIERRRRNKMTAYITELSDMVPTCSALARKPDKLTILRMAVSHMKSLRGTGNTSTDGSYKPSFLTDQELKHLILEAADGFLFIVSCETGRVVYVSDSVTPVLNQQQSEWFGSTLYDQVHPDDVDKLREQLSTSENALTGRILDLKTGTVKKEGQQSSMRMCMGSRRSFICRMRCGSSSVDPVSVNRLSFVRNRCRNGLGSVKDGEPHFVVVHCTGYIKAWPPAGVSLPDDDPEAGQGSKFCLVAIGRLQVTSS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 aryl hydrocarbon receptor nuclear translocator
别名 ARNT/HIF-1 beta, ARNT, HIF-1 beta, HIF1B, HIF 1 beta
计算分子量 416 aa, 46 kDa, 87 kDa
观测分子量 87 kDa
GenBank蛋白编号BC060838
基因名称 ARNT/HIF-1 beta
Gene ID (NCBI) 405
RRIDAB_2883619
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDP27540
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIF1B, also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia

实验方案

Product Specific Protocols
FC protocol for CL594 ARNT,HIF1B antibody CL594-66732Download protocol
IF protocol for CL594 ARNT,HIF1B antibody CL594-66732Download protocol
Standard Protocols
Click here to view our Standard Protocols
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