×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 24011-1-AP

    WB analysis using 24011-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24011-1-AP (ARMC2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse testis using 24011-1-AP

    IF Staining of mouse testis using 24011-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using ARMC2 antibody (24011-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 24011-1-AP

    IF Staining of mouse brain using 24011-1-AP

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using ARMC2 antibody (24011-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ARMC2 Polyclonal antibody

ARMC2 Polyclonal Antibody for WB, IF-Fro, ELISA
Cat No. 24011-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-Fro, ELISA

别名

armadillo repeat containing 2, ARMC 2, RP11 787I22.1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, mouse testis tissue, rat testis tissue
Positive IF-Fro detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24011-1-AP targets ARMC2 in WB, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-Fro, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag21162

Product name: Recombinant human ARMC2 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 510-860 aa of BC030603

Sequence: CYALFLNLINKYQKKQDLVVRVVFILGNLTAKNNQAREQFSKEKGSIQTLLSLFQTFHQLDLHSQKPVGQRGEQHRAQRPPSEAEDVLIKLTRVLANIAIHPGVGPVLAANPGIVGLLLTTLEYKSLDDCEELVINATATINNLSYYQVKNSIIQDKKLYIAELLLKLLVSNNMDGILEAVRVFGNLSQDHDVCDFIVQNNVHRFMMALLDAQHQDICFSACGVLLNLTVDKDKRVILKEGGGIKKLVDCLRDLGPTDWQLACLVCKTLWNFSENITNASSCFGNEDTNTLLLLLSSFLDEELALDGSFDPDLKNYHKLHWETEFKPVAQQLLNRIQRHHTFLEPLPIPSF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 armadillo repeat containing 2
别名 armadillo repeat containing 2, ARMC 2, RP11 787I22.1
计算分子量 867 aa, 97 kDa
观测分子量78 kDa,97 kDa
GenBank蛋白编号BC030603
基因名称 ARMC2
Gene ID (NCBI) 84071
RRIDAB_3669428
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ8NEN0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ARMC2 protein (Armadillo Repeat Containing 2) is an evolutionarily conserved axonemal-related protein that plays a crucial role in the structure and function of cilia and flagella. It interacts with the kinesin-2 complex through its unique Armadillo repeat domain and acts as an adapter for the flagellar transport (IFT) process, responsible for transporting specific cargoes (such as the precursor proteins necessary for the construction of flagellar axons) from the cell body to the flagellar tip, which is essential for the assembly, maintenance, and signal transduction functions of the flagella. Its dysfunction is directly related to abnormal sperm flagellar morphology and movement disorders.

实验方案

Product Specific Protocols
IF protocol for ARMC2 antibody 24011-1-APDownload protocol
WB protocol for ARMC2 antibody 24011-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...