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验证数据展示

  • WB analysis using 68186-1-Ig

    WB analysis using 68186-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A/ARL8B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis using 68186-1-Ig

    WB analysis using 68186-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A/ARL8B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of C6 using 68186-1-Ig

    IF Staining of C6 using 68186-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using ARL8A/ARL8B antibody (68186-1-Ig, Clone: 2F4C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of Neuro-2a using 68186-1-Ig

    IF Staining of Neuro-2a using 68186-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using ARL8A/ARL8B antibody (68186-1-Ig, Clone: 2F4C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of A549 using 68186-1-Ig

    IF Staining of A549 using 68186-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ARL8A/ARL8B antibody (68186-1-Ig, Clone: 2F4C3 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

  • IF Staining of HEK-293 using 68186-1-Ig

    IF Staining of HEK-293 using 68186-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using ARL8A/ARL8B antibody (68186-1-Ig, Clone: 2F4C3 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

  • WB analysis using 68186-1-Ig

    WB analysis using 68186-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

ARL8A/ARL8B Monoclonal antibody

ARL8A/ARL8B Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68186-1-Ig

产品说明书

CloneNo. 2F4C3

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, rabbit

应用

WB, IF/ICC, ELISA

别名

ARL8A, GIE2, ARL10B, ADP-ribosylation factor-like protein 8A, ADP-ribosylation factor-like protein 10B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inrabbit brain tissue, LNCaP cells, human placenta tissue, HeLa cells, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inA549 cells, C6 cells, Neuro-2a cells, HEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy ARL8A/ARL8B Ready-To-Use IHC Kit. ARL8A/ARL8B primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68186-1-Ig targets ARL8A/ARL8B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, rabbit
免疫原

CatNo: Ag10758

Product name: Recombinant human ARL8A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-186 aa of BC015408

Sequence: MIALFNKLLDWFKALFWKEEMELTLVGLQYSGKTTFVNVIASGQFNEDMIPTVGFNMRKITKGNVTIKLWDIGGQPRFRSMWERYCRGVSAIVYMVDAADQEKIEASKNELHNLLDKPQLQGIPVLVLGNKRDLPGALDEKELIEKMNLSAIQDREICCYSISCKEKDNIDITLQWLIQHSKSRRS

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ADP-ribosylation factor-like 8A
别名 ARL8A, GIE2, ARL10B, ADP-ribosylation factor-like protein 8A, ADP-ribosylation factor-like protein 10B
计算分子量 186 aa, 21 kDa
观测分子量18 kDa, 21 kDa
GenBank蛋白编号BC015408
基因名称 ARL8A
Gene ID (NCBI) 127829
RRIDAB_3085047
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96BM9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARL8A (ADP-ribosylation factor-like protein 8A) is also named as ARL10B, GIE2 and belongs to the small GTPase superfamily. ARL8A is localized to lysosomes in mammalian cells (PMID: 16537643). In neurons, ARL8A mediates the anterograde axonal long-range transport of presynaptic lysosome-related vesicles that are required for presynaptic biogenesis and synaptic function. ARL8A is upregulated in many cancers such as endometrial cancer (PMID: 32070877), colorectal cancer (PMID: 30546056) and inflammatory breast cancer (PMID: 27648361), and has been associated with poor survival outcomes. ARL8A/ARL8B can be recognized by this antibody.

实验方案

Product Specific Protocols
IF protocol for ARL8A/ARL8B antibody 68186-1-IgDownload protocol
WB protocol for ARL8A/ARL8B antibody 68186-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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