验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, K-562 cells, HeLa cells, mouse liver tissue, HepG2 cells, Neuro-2a cells, C6 cells |
| Positive IP detected in | Neuro-2a cells |
| Positive IHC detected in | human stomach cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16012-1-AP targets ARL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag8770 Product name: Recombinant human ARL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-181 aa of BC007000 Sequence: MGGFFSSIFSSLFGTREMRILILGLDGAGKTTILYRLQVGEVVTTIPTIGFNVETVTYKNLKFQVWDLGGQTSIRPYWRCYYSNTDAVIYVVDSCDRDRIGISKSELVAMLEEEELRKAILVVFANKQDMEQAMTSSEMANSLGLPALKDRKWQIFKTSATKGTGLDEAMEWLVETLKSRQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ADP-ribosylation factor-like 1 |
| 别名 | ADP ribosylation factor like 1, ADP-ribosylation factor-like protein 1 |
| 计算分子量 | 181 aa, 20 kDa |
| 观测分子量 | 20 kDa |
| GenBank蛋白编号 | BC007000 |
| 基因名称 | ARL1 |
| Gene ID (NCBI) | 400 |
| RRID | AB_2243131 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P40616 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ARL1 antibody 16012-1-AP | Download protocol |
| IHC protocol for ARL1 antibody 16012-1-AP | Download protocol |
| IP protocol for ARL1 antibody 16012-1-AP | Download protocol |
| WB protocol for ARL1 antibody 16012-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Protoc Proteome-wide analysis of protein lipidation using chemical probes: in-gel fluorescence visualization, identification and quantification of N-myristoylation, N- and S-acylation, O-cholesterylation, S-farnesylation and S-geranylgeranylation. | ||
Nat Commun Rab27a co-ordinates actin-dependent transport by controlling organelle-associated motors and track assembly proteins. | ||
J Cell Biol ARFRP1 functions upstream of ARL1 and ARL5 to coordinate recruitment of distinct tethering factors to the trans-Golgi network.
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