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验证数据展示

  • FC experiment of U2OS using CL488-83489

    FC experiment of U2OS using CL488-83489

    1x10^6 U2OS cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated ARID4A Recombinant monoclonal antibody (CL488-83489, Clone:240436A4)(red), or 0.8 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated ARID4A Recombinant monoclonal antibody

ARID4A Uni-rAb® Recombinant Antibody for FC (Intra)
Cat No. CL488-83489

产品说明书

CloneNo. 240436A4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

240436A4, ARID 4A, ARID domain-containing protein 4A, AT-rich interactive domain-containing protein 4A, RBBP 1

缓冲液配方:  PBS, Proclin300, BSA, Glycerol
PBS, Proclin300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inU2OS cells
For other applications, we recommend the unconjugated version of this antibody, 83489-1-PBS

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.8 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83489 targets ARID4A in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Ag33114

Product name: Recombinant human ARID4A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 900-1105 aa of NM_002892.3

Sequence: FEQHFERENEGMPSLIAESNQCIQQLTSERFDSPAEETVNIPLKEDEDAMPLIGPETLVCHEVDLDDLDEKDKTSIEDVAVESSESNSLVSIPPALPPVVQHNFSVASPLTLSQDESRSVKSESDITIEVDSIAEESQEGLCERESANGFETNVASGTCSIIVQERESREKGQKRPSDGNSGLMAKKQKRTPKRTSAAAKNEKNGT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 AT rich interactive domain 4A (RBP1-like)
别名 240436A4, ARID 4A, ARID domain-containing protein 4A, AT-rich interactive domain-containing protein 4A, RBBP 1
计算分子量143 kDa
观测分子量205 kDa
GenBank蛋白编号NM_002892.3
基因名称 ARID4A
Gene ID (NCBI) 5926
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
激发激光Blue laser (488 nm)
形式Liquid
纯化方式Protein A purification
UNIPROT IDP29374
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARID4A (AT-rich interaction domain 4A, also known as retinoblastoma-binding protein 1, RBBP1/RBP1) is a retinoblastoma (RB)-binding protein. ARID1A is an important subunit of the switching defective/sucrose nonfermenting (SWI/SNF) chromatin remodeling complex.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 ARID4A antibody CL488-83489Download protocol
Standard Protocols
Click here to view our Standard Protocols
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