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ARHGDIG Polyclonal antibody

ARHGDIG Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 14282-1-AP

Print datasheet

Synonyms

ARHGDIG, Rho GDI 3, Rho GDI gamma, RHOGDI 3

验证数据展示

  • WB analysis using 14282-1-AP

    WB analysis using 14282-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14282-1-AP (ARHGDIG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 14282-1-AP

    IHC staining of human pancreas cancer using 14282-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14282-1-AP (ARHGDIG antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human pancreas cancer using 14282-1-AP

    IHC staining of human pancreas cancer using 14282-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14282-1-AP (ARHGDIG antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inHEK-293 cells, human brain tissue, HeLa cells, Jurkat cells, MCF-7 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14282-1-AP targets ARHGDIG in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ARHGDIG fusion protein Ag5554 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rho GDP dissociation inhibitor (GDI) gamma
Synonyms ARHGDIG, Rho GDI 3, Rho GDI gamma, RHOGDI 3
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC047699
Gene Symbol ARHGDIG
Gene ID (NCBI) 398
RRIDAB_2059733
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99819
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ARHGDIG antibody 14282-1-APDownload protocol
IHC protocol for ARHGDIG antibody 14282-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncol Rep

Upregulation and hypomethylation of lncRNA AFAP1‑AS1 predicts a poor prognosis and promotes the migration and invasion of cervical cancer.

Authors - Hao Bo
View Article