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验证数据展示

  • WB analysis using 14282-1-AP

    WB analysis using 14282-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14282-1-AP (ARHGDIG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 14282-1-AP

    IHC staining of human pancreas cancer using 14282-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14282-1-AP (ARHGDIG antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human pancreas cancer using 14282-1-AP

    IHC staining of human pancreas cancer using 14282-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14282-1-AP (ARHGDIG antibody) at dilution of 1:100 (under 40x lens).

ARHGDIG Polyclonal antibody

ARHGDIG Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14282-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ARHGDIG, Rho GDI 3, Rho GDI gamma, RHOGDI 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, human brain tissue, HeLa cells, Jurkat cells, MCF-7 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14282-1-AP targets ARHGDIG in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag5554

Product name: Recombinant human ARHGDIG protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-225 aa of BC047699

Sequence: MLGLDACELGAQLLELLRLALCARVLLADKEGGPPAVDEVLDEAVPEYRAPGRKSLLEIRQLDPDDRSLAKYKRVLLGPLPPAVDPSLPNVQVTRLTLLSEQAPGPVVMDLTGDLAVLKDQVFVLKEGVDYRVKISFKVHREIVSGLKCLHHTYRRGLRVDKTVYMVGSYGPSAQEYEFVTPVEEAPRGALVRGPYLVVSLFTDDDRTHHLSWEWGLCICQDWKD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rho GDP dissociation inhibitor (GDI) gamma
别名 ARHGDIG, Rho GDI 3, Rho GDI gamma, RHOGDI 3
计算分子量 25 kDa
观测分子量 25 kDa
GenBank蛋白编号BC047699
基因名称 ARHGDIG
Gene ID (NCBI) 398
RRIDAB_2059733
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99819
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ARHGDIG antibody 14282-1-APDownload protocol
IHC protocol for ARHGDIG antibody 14282-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncol Rep

Upregulation and hypomethylation of lncRNA AFAP1‑AS1 predicts a poor prognosis and promotes the migration and invasion of cervical cancer.

Authors - Hao Bo
View Article