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验证数据展示

  • WB analysis using 32382-1-AP (same clone as 32382-1-PBS)

    WB analysis using 32382-1-AP (same clone as 32382-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32382-1-AP (ARHGAP44 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32382-1-PBS in a different storage buffer formulation.

  • WB analysis using 32382-1-AP (same clone as 32382-1-PBS)

    WB analysis using 32382-1-AP (same clone as 32382-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32382-1-AP (ARHGAP44 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32382-1-PBS in a different storage buffer formulation.

  • IHC staining of human urothelial carcinoma using 32382-1-AP (same clone as 32382-1-PBS)

    IHC staining of human urothelial carcinoma using 32382-1-AP (same clone as 32382-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 32382-1-AP (ARHGAP44 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32382-1-PBS in a different storage buffer formulation.

  • IHC staining of human urothelial carcinoma using 32382-1-AP (same clone as 32382-1-PBS)

    IHC staining of human urothelial carcinoma using 32382-1-AP (same clone as 32382-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 32382-1-AP (ARHGAP44 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32382-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 32382-1-AP (same clone as 32382-1-PBS)

    IF Staining of HEK-293 using 32382-1-AP (same clone as 32382-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ARHGAP44 antibody (32382-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 32382-1-PBS in a different storage buffer formulation.

ARHGAP44 Polyclonal antibody, PBS Only

ARHGAP44 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 32382-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

KIAA0672, NPC-A-10, Rho GTPase Activating Protein 44, Rho GTPase-activating protein 44, Rho GTPase-Activating Protein RICH2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

32382-1-PBS targets ARHGAP44 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag38196

Product name: Recombinant human ARHGAP44 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 580-772 aa of NM_001321166

Sequence: LQPGPERTSTTKSKELSPGSAQKGSPGSSQGTACAGTQPGAQPGAQPGASPSPSQPPADQSPHTLRKVSKKLAPIPPKVPFGQPGAMADQSAGQPSPVSLSPTPPSTPSPYGLSYPQGYSLASGQLSPAAAPPLASPSVFTSTLSKSRPTPKPRQRPTLPPPQPPTVNLSASSPQSTEAPMLDGMSPGESMST

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rho-type GTPase-activating protein RICH2
别名 KIAA0672, NPC-A-10, Rho GTPase Activating Protein 44, Rho GTPase-activating protein 44, Rho GTPase-Activating Protein RICH2
计算分子量818aa, 89 kDa
观测分子量80-100 kDa
GenBank蛋白编号NM_001321166
基因名称 RICH2
Gene ID (NCBI) 9912
RRIDAB_3742591
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ17R89
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Rho GTPase-activating protein 44 (ARHGAP44) belongs to the Rho GTPase-activating protein (RhoGAP) family, also known as Rich2.ARHGAP44 plays an important role in cytoskeletal dynamics, cellular motility and signaling. Its high expression in osteosarcoma correlates with tumor malignancy, suggesting its potential as a potential therapeutic target. In addition, ARHGAP44 may also be involved in the pathogenesis of diabetes and cardiovascular diseases, and its specific role in these diseases needs to be further investigated in the future.

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