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验证数据展示

  • WB analysis using 32382-1-AP

    WB analysis using 32382-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32382-1-AP (ARHGAP44 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

ARHGAP44 Polyclonal antibody

ARHGAP44 Polyclonal Antibody for WB, ELISA
Cat No. 32382-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

RhoGAP interacting with CIP4 homologs protein 2, Rho GTPase-Activating Protein RICH2, Rho GTPase-activating protein 44, Rho GTPase Activating Protein 44, NPC-A-10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, LNCaP cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32382-1-AP targets ARHGAP44 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 ARHGAP44 fusion protein Ag38196 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rho-type GTPase-activating protein RICH2
别名 RhoGAP interacting with CIP4 homologs protein 2, Rho GTPase-Activating Protein RICH2, Rho GTPase-activating protein 44, Rho GTPase Activating Protein 44, NPC-A-10
计算分子量818aa, 89 kDa
观测分子量80-100 kDa
GenBank蛋白编号NM_001321166
基因名称 RICH2
Gene ID (NCBI) 9912
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ17R89
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rho GTPase-activating protein 44 (ARHGAP44) belongs to the Rho GTPase-activating protein (RhoGAP) family, also known as Rich2.ARHGAP44 plays an important role in cytoskeletal dynamics, cellular motility and signaling. Its high expression in osteosarcoma correlates with tumor malignancy, suggesting its potential as a potential therapeutic target. In addition, ARHGAP44 may also be involved in the pathogenesis of diabetes and cardiovascular diseases, and its specific role in these diseases needs to be further investigated in the future.

实验方案

Product Specific Protocols
WB protocol for ARHGAP44 antibody 32382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols