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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85648-2-RR (same clone as 85648-2-PBS)

    WB analysis using 85648-2-RR (same clone as 85648-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85648-2-RR (ARHGAP4 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85648-2-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 85648-2-RR (same clone as 85648-2-PBS)

    IF Staining of Jurkat using 85648-2-RR (same clone as 85648-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ARHGAP4 antibody (85648-2-RR, Clone: 242995B5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85648-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85648-2-RR

    Affinity and Kinetic Characterization of 85648-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85648-2-RR against Human ARHGAP4 were performed. The affinity constant is 0.518 nM.

ARHGAP4 Recombinant antibody, PBS Only

ARHGAP4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85648-2-PBS

产品说明书

CloneNo. 242995B5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

KIAA0131, p115, RGC1, Rho GTPase-activating protein 4, Rho-GAP hematopoietic protein C1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ARHGAP4 Ready-To-Use IHC Kit. ARHGAP4 primary antibody included.

产品信息

85648-2-PBS targets ARHGAP4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 ARHGAP4 fusion protein Ag10091 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Rho GTPase activating protein 4
别名 KIAA0131, p115, RGC1, Rho GTPase-activating protein 4, Rho-GAP hematopoietic protein C1
计算分子量 986 aa, 109 kDa
观测分子量 115-120 kDa
GenBank蛋白编号BC052303
基因名称 ARHGAP4
Gene ID (NCBI) 393
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP98171
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ARHGAP4 (Rho GTPase-Activating Protein 4), also named as KIAA0131, RGC1, RHOGAP4, is a member of the RhoGAP family, which regulates Rho GTPases by accelerating their hydrolysis of GTP to GDP, thereby inactivating these signaling molecules. Rho GTPases are critical regulators of cytoskeletal dynamics, cell motility, and intracellular trafficking.