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验证数据展示

  • WB analysis using 85648-2-RR

    WB analysis using 85648-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85648-2-RR (ARHGAP4 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 85648-2-RR

    IF Staining of Jurkat using 85648-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ARHGAP4 antibody (85648-2-RR, Clone: 242995B5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 85648-2-RR

    Affinity and Kinetic Characterization of 85648-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85648-2-RR against Human ARHGAP4 were performed. The affinity constant is 0.518 nM.

ARHGAP4 Recombinant antibody

ARHGAP4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85648-2-RR

产品说明书

CloneNo. 242995B5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

KIAA0131, p115, RGC1, Rho GTPase-activating protein 4, Rho-GAP hematopoietic protein C1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, K-562 cells
Positive IF/ICC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy ARHGAP4 Ready-To-Use IHC Kit. ARHGAP4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85648-2-RR targets ARHGAP4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 ARHGAP4 fusion protein Ag10091 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Rho GTPase activating protein 4
别名 KIAA0131, p115, RGC1, Rho GTPase-activating protein 4, Rho-GAP hematopoietic protein C1
计算分子量 986 aa, 109 kDa
观测分子量 115-120 kDa
GenBank蛋白编号BC052303
基因名称 ARHGAP4
Gene ID (NCBI) 393
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP98171
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARHGAP4 (Rho GTPase-Activating Protein 4), also named as KIAA0131, RGC1, RHOGAP4, is a member of the RhoGAP family, which regulates Rho GTPases by accelerating their hydrolysis of GTP to GDP, thereby inactivating these signaling molecules. Rho GTPases are critical regulators of cytoskeletal dynamics, cell motility, and intracellular trafficking.

实验方案

Product Specific Protocols
WB protocol for ARHGAP4 antibody 85648-2-RRDownload protocol
IF protocol for ARHGAP4 antibody 85648-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols