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验证数据展示

  • WB analysis using 31277-1-AP (same clone as 31277-1-PBS)

    WB analysis using 31277-1-AP (same clone as 31277-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31277-1-AP (ARFGEF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31277-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 31277-1-AP (same clone as 31277-1-PBS)

    IHC staining of human stomach cancer using 31277-1-AP (same clone as 31277-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31277-1-AP (ARFGEF2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31277-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARFGEF2 antibody included
  • IHC staining of human stomach cancer using 31277-1-AP (same clone as 31277-1-PBS)

    IHC staining of human stomach cancer using 31277-1-AP (same clone as 31277-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31277-1-AP (ARFGEF2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31277-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARFGEF2 antibody included

ARFGEF2 Polyclonal antibody, PBS Only

ARFGEF2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 31277-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

BIG2, Brefeldin A inhibited GEP 2, ARFGEP2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® ARFGEF2 Ready-To-Use IHC Kit. ARFGEF2 primary antibody included.

产品信息

31277-1-PBS targets ARFGEF2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag35396

Product name: Recombinant human ARFGEF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 211-370 aa of BC050449

Sequence: PIQSKPQSPVIQAAAVSPKFVRLKHSQAQSKPTTPEKTDLTNGEHARSDSGKVSTENGDAPRERGSSLSGTDDGAQEVVKDILEDVVTSAIKEAAEKHGLTEPERVLGELECQECAIPPGVDENSQTNGIADDRQSLSSADNLESDAQGHQVAARFSHVL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
别名 BIG2, Brefeldin A inhibited GEP 2, ARFGEP2
观测分子量202 kDa
GenBank蛋白编号BC050449
基因名称 ARFGEF2
Gene ID (NCBI) 10564
RRIDAB_3669925
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6D5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. ARFGEF2 is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. ARFGEF2 contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition.

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