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验证数据展示

  • WB analysis using 31684-1-AP (same clone as 31684-1-PBS)

    WB analysis using 31684-1-AP (same clone as 31684-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31684-1-AP (ARFGEF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31684-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 31684-1-AP (same clone as 31684-1-PBS)

    IHC staining of human ovary cancer using 31684-1-AP (same clone as 31684-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31684-1-AP (ARFGEF1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31684-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARFGEF1 antibody included

ARFGEF1 Polyclonal antibody, PBS Only

ARFGEF1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 31684-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

p200 ARF GEP1, P200, Brefeldin A inhibited GEP 1, BIG1, ARFGEP1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® ARFGEF1 Ready-To-Use IHC Kit. ARFGEF1 primary antibody included.

产品信息

31684-1-PBS targets ARFGEF1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag35629

Product name: Recombinant human ARFGEF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 212-420 aa of NM_001413184

Sequence: QALQEAKQMEKERHRQHHHLLQSPVSHHEPESPQLRYLPPQTVDHISQEHEGDLDLHTNDVDKSLQDDTEPENGSDISSAENEQTEADQATAAETLSKNEVLYDGENHDCEEKPQDIVQNIVEEMVNIVVGDMGEGTTINASADGNIGTIEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKFSHILQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited)
别名 p200 ARF GEP1, P200, Brefeldin A inhibited GEP 1, BIG1, ARFGEP1
计算分子量208kDa
观测分子量210 kDa
GenBank蛋白编号NM_001413184
基因名称 ARFGEF1
Gene ID (NCBI) 10565
RRIDAB_3670078
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9Y6D6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ARFGEF1 (ADP ribosylation factor guanine nucleotide exchange factor 1), also known as BIG1 (Brefeldin A-inhibited guanine nucleotide exchange protein 1), is one of the ADP ribosylation factor (ARF) guanine nucleotide exchange factor (GEF) with a conserved catalytic sec7 domain.ARFGEF1 is connected with trafficking regulation through the trans-Golgi network and endosomes (PMID: 35090882).

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