验证数据展示
经过测试的应用
| Positive IF/ICC detected in | SH-SY5Y cells |
Planning an IHC experiment? We recommend our IHCeasy ARC Ready-To-Use IHC Kit. ARC primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66550-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66550 targets ARC in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig, rabbit samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, Mouse, Rat, Pig, rabbit |
| 免疫原 | ARC fusion protein Ag9445 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | activity-regulated cytoskeleton-associated protein |
| 别名 | ARC, ARC/ARG3.1, Arg3.1, KIAA0278 |
| 计算分子量 | 396 aa, 45 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC012321 |
| 基因名称 | ARC |
| Gene ID (NCBI) | 23237 |
| RRID | AB_2934478 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q7LC44 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARC, also named as KIAA0278 and Arg3.1, mediates endocytosis of neuronal AMPA-type glutamate receptors (AMPARs). It is required for consolidation of synaptic plasticity as well as formation of long-term memory. ARC plays a role in the regulation of cell morphology and cytoskeletal organization. It is required in the stress fiber dynamics and cell migration. Recent study shows that expression of Arc can regulate cognitive flexibility.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ARC antibody CL488-66550 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |