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APTX Polyclonal antibody

APTX Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 26747-1-AP

Print datasheet

Synonyms

AOA, AOA1, aprataxin, APTX, AXA1, EAOH, EOAHA, FHA HIT

验证数据展示

  • IF Staining of HepG2 using 26747-1-AP

    IF Staining of HepG2 using 26747-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 26747-1-AP (APTX antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of HEK-293T using 26747-1-AP

    WB analysis of HEK-293T using 26747-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 26747-1-AP (APTX Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293T cells, HepG2 cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26747-1-AP is APTX Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB,ELISA
Tested Reactivity human
Immunogen APTX fusion protein Ag24945 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name aprataxin
Synonyms AOA, AOA1, aprataxin, APTX, AXA1, EAOH, EOAHA, FHA HIT
Calculated Molecular Weight 342 aa, 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC104881
Gene Symbol APTX
Gene ID (NCBI) 54840
RRIDAB_2880620
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z2E3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

APTX, also named as Aprataxin, is a nuclear protein, present in both the nucleoplasm and the nucleolus, which is a member of the histidine triad (HIT) superfamily. APTX is involved in DNA single-strand break repair, mediating protein-protein interactions with molecules responding to DNA damage. APTX contains three conserved domains: an N-terminal forkhead-associated (FHA) domain which mediates protein-protein interactions, a HIT domain that is similar to Hint, and a C-terminal zinc finger domain. Loss of function mutations in APTX, the gene encoding for Aprataxin, destabilize the Aprataxin protein and result in a rare neurological disorder known as ataxia-oculomotor apraxia, characterized by abnormal movements of the head and eyes.

实验方案

Product Specific Protocols
WB protocol for APTX antibody 26747-1-APDownload protocol
IF protocol for APTX antibody 26747-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols