APP Polyclonal antibody
APP Polyclonal Antibody for IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
10524-1-AP targets APP in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | IHC, ELISA |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Immunogen | APP fusion protein Ag0769 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | amyloid beta (A4) precursor protein |
| Synonyms | A4, AAA, ABETA, ABPP, AD1, AICD 50, AICD 57, AICD 59, AID(50), AID(57), AID(59), Amyloid beta A4 protein, amyloid P, amyloid precursor protein, Amyloid-beta precursor protein, APP, APPI, beta Amyloid, Beta-amyloid protein 40, Beta-amyloid protein 42, Beta-APP42, CTFgamma, CVAP, Gamma CTF(50) C31, PN II, PN2, PreA4, Protease nexin II, S APP alpha Soluble APP beta |
| Calculated Molecular Weight | 87 kDa |
| GenBank Accession Number | BC004369 |
| Gene Symbol | APP |
| Gene ID (NCBI) | 351 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05067 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Aβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1). This antibody can recogniaze the N-terminus of human APP: Soluble APP-alpha and Soluble APP-beta.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for APP antibody 10524-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neurobiol Aging Beta amyloid-induced upregulation of death receptor 6 accelerates the toxic effect of N-terminal fragment of amyloid precursor protein. | ||
Int J Biol Macromol Polysaccharides from Pleurotus ostreatus alleviate cognitive impairment in a rat model of Alzheimer's disease. | ||
Neurotoxicology Protective mechanism of gold nanoparticles on human neural stem cells injured by β-amyloid protein through miR-21-5p/SOCS6 pathway |