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验证数据展示

  • WB analysis using 28514-1-AP (same clone as 28514-1-PBS)

    WB analysis using 28514-1-AP (same clone as 28514-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28514-1-AP (APOOL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28514-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 28514-1-AP (same clone as 28514-1-PBS)

    IHC staining of human kidney using 28514-1-AP (same clone as 28514-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28514-1-AP (APOOL antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28514-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with APOOL antibody included
  • IHC staining of human kidney using 28514-1-AP (same clone as 28514-1-PBS)

    IHC staining of human kidney using 28514-1-AP (same clone as 28514-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28514-1-AP (APOOL antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28514-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with APOOL antibody included
  • IHC staining of human placenta using 28514-1-AP (same clone as 28514-1-PBS)

    IHC staining of human placenta using 28514-1-AP (same clone as 28514-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28514-1-AP (APOOL antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28514-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with APOOL antibody included
  • IHC staining of human placenta using 28514-1-AP (same clone as 28514-1-PBS)

    IHC staining of human placenta using 28514-1-AP (same clone as 28514-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28514-1-AP (APOOL antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28514-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with APOOL antibody included
  • IF Staining of HeLa using 28514-1-AP (same clone as 28514-1-PBS)

    IF Staining of HeLa using 28514-1-AP (same clone as 28514-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using APOOL antibody (28514-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28514-1-PBS in a different storage buffer formulation.

APOOL Polyclonal antibody, PBS Only

APOOL Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28514-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy APOOL Ready-To-Use IHC Kit. APOOL primary antibody included.

产品信息

28514-1-PBS targets APOOL in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag29392

Product name: Recombinant human APOOL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 127-268 aa of BC107096

Sequence: VSARKGSKFKKITYPLGLATLGATVCYPVQSVIIAKVTAKKVYATSQQIFGAVKSLWTKSSKEESLPKPKEKTKLGSSSEIEVPAKTTHVLKHSVPLPTELSSEAKTKSESTSGATQFMPDPKLMDHGQSHPEDIDMYSTRS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 apolipoprotein O-like
别名
计算分子量 268 aa, 29 kDa
观测分子量30 kDa
GenBank蛋白编号BC107096
基因名称 APOOL
Gene ID (NCBI) 139322
RRIDAB_3086061
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UXV4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

APOOL, the component of the MICOS complex, is a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Specifically binds to cardiolipin (in vitro) but not to the precursor lipid phosphatidylglycerol. Plays a crucial role in crista junction formation and mitochondrial function (PubMed:23704930), (PubMed:25764979). Its molecular weight is about 30 kDa.

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