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验证数据展示

  • IF Staining of human liver using CL647-66124

    IF Staining of human liver using CL647-66124

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CL647-66124 (APOL1-Specific antibody) at dilution of 1:50.

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  • KD/KO验证

CoraLite® Plus 647-conjugated APOL1-Specific Monoclonal antibody

APOL1-Specific Monoclonal Antibody for IF-P
Cat No. CL647-66124

产品说明书

CloneNo. 1G12D11

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IF-P

别名

APOL1, Apolipoprotein L-I, Apolipoprotein L I, ApoL-I, Apo-L

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman liver tissue
Planning an IHC experiment? We recommend our IHCeasy APOL1 Ready-To-Use IHC Kit. APOL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66124-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-66124 targets APOL1-Specific in IF-P applications and shows reactivity with human samples.

经测试应用 IF-P Application Description
经测试反应性 human
免疫原 APOL1-Specific fusion protein Ag2016 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 apolipoprotein L, 1
别名 APOL1, Apolipoprotein L-I, Apolipoprotein L I, ApoL-I, Apo-L
计算分子量 44 kDa
GenBank蛋白编号BC017331
基因名称 APOL1
Gene ID (NCBI) 8542
RRIDAB_2883731
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO14791
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue. This antibody is conjugated with CL647, Ex/Em 650 nm/665 nm.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 APOL1-Specific antibody CL647-66124Download protocol
Standard Protocols
Click here to view our Standard Protocols