CoraLite® Plus 647-conjugated APOE Monoclonal antibody
APOE Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
1B2C9
验证数据展示
经过测试的应用
Positive FC detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 66830-1-Ig
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-66830 targets APOE in FC (Intra) applications and shows reactivity with human, mouse samples.
Tested Applications | FC (Intra) |
Tested Reactivity | human, mouse |
Immunogen | APOE fusion protein Ag28186 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | apolipoprotein E |
Synonyms | AD2, Apo E, APOE, apolipoprotein E, LPG |
Calculated Molecular Weight | 36 kDa |
Observed Molecular Weight | 34-36 kDa |
GenBank Accession Number | BC003557 |
Gene Symbol | APOE |
Gene ID (NCBI) | 348 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P02649 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serve as a ligand for the LDL (APO B/E) receptor and for the specific APOE receptor (chylomicron remnant) of hepatic tissues.
The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight.
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 647 APOE antibody CL647-66830 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |