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APOE Polyclonal antibody
APOE Polyclonal Antibody for WB, IF/ICC, FC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF/ICC, FC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HuH-7 cells, HepG2 cells, human plasma, mouse serum |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
18254-1-AP targets APOE in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IF/ICC, FC, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | APOE fusion protein Ag13070 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | apolipoprotein E |
Synonyms | AD2, Apo E, APOE, apoe4, apolipoprotein E, LPG |
Calculated Molecular Weight | 36 kDa |
Observed Molecular Weight | 34-38 kDa |
GenBank Accession Number | BC003557 |
Gene Symbol | APOE |
Gene ID (NCBI) | 348 |
RRID | AB_2878525 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P02649 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for APOE antibody 18254-1-AP | Download protocol |
IF protocol for APOE antibody 18254-1-AP | Download protocol |
FC protocol for APOE antibody 18254-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Br J Cancer A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline. | ||
J Genet Genomics Comprehensive lipidomics in apoM-/- mice reveals an overall state of metabolic distress and attenuated hepatic lipid secretion into the circulation. | ||
Hum Cell Serine protease HtrA2/Omi regulates adaptive mitochondrial reprogramming in the brain cortex after ischemia/reperfusion injury via UCP2-SIRT3-PGC1 axis. | ||
Pulm Pharmacol Ther Protective effects of adipose-derived biogenic nanoparticles on the pulmonary microvascular endothelial barrier in mice with ventilator-induced lung injury via the TRPV4/ROCK1 signalling pathway. | ||
Autoimmunity Inclisiran inhibits oxidized low-density lipoprotein-induced foam cell formation in Raw264.7 macrophages via activating the PPARγ pathway. |