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验证数据展示

  • WB analysis using 13384-1-AP (same clone as 13384-1-PBS)

    WB analysis using 13384-1-AP (same clone as 13384-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13384-1-AP (AP3B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13384-1-PBS in a different storage buffer formulation.

  • IP experiment of COLO 320 using 13384-1-AP (same clone as 13384-1-PBS)

    IP experiment of COLO 320 using 13384-1-AP (same clone as 13384-1-PBS)

    IP result of anti-AP3B1 (IP:13384-1-AP, 3ug; Detection:13384-1-AP 1:500) with COLO 320 cells lysate 2500ug. This data was developed using the same antibody clone with 13384-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 13384-1-AP (same clone as 13384-1-PBS)

    IHC staining of rat brain using 13384-1-AP (same clone as 13384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13384-1-AP (AP3B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13384-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AP3B1 antibody included
  • IHC staining of rat brain using 13384-1-AP (same clone as 13384-1-PBS)

    IHC staining of rat brain using 13384-1-AP (same clone as 13384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13384-1-AP (AP3B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13384-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AP3B1 antibody included
  • Featured Product
  • KD/KO Validated

AP3B1 Polyclonal antibody, PBS Only

AP3B1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 13384-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® AP3B1 Ready-To-Use IHC Kit. AP3B1 primary antibody included.

产品信息

13384-1-PBS targets AP3B1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4225

Product name: Recombinant human AP3B1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 763-1094 aa of BC038444

Sequence: KTSDSSNDESSSIEDSSSDSESESEPESESESRRVTKEKEKKTKQDRTPLTKDVSLLDLDDFNPVSTPVALPTPALSPSLMADLEGLHLSTSSSVISVSTPAFVPTKTHVLLHRMSGKGLAAHYFFPRQPCIFGDKMVSIQITLNNTTDRKIENIHIGEKKLPIGMKMHVFNPIDSLEPEGSITVSMGIDFCDSTQTASFQLCTKDDCFNVNIQPPVGELLLPVAMSEKDFKKEQGVLTGMNETSAVIIAAPQNFTPSVIFQKVVNVANVGAVPSGQDNIHRFAAKTVHSGSLMLVTVELKEGSTAQLIINTEKTVIGSVLLRELKPVLSQG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adaptor-related protein complex 3, beta 1 subunit
别名
计算分子量 1094 aa, 121 kDa
观测分子量 140 kDa
GenBank蛋白编号BC038444
基因名称 AP3B1
Gene ID (NCBI) 8546
RRIDAB_2056499
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00203
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AP3B1 is the 140-kDa β3A subunit of the adaptor-related protein complex-3 (AP-3), a ubiquitous heterotetrameric complex that is localized to the trans-Golgi network and endosomes and is involved in protein trafficking to lysosomes or specialized endosomal-lysosomal organelles (PMID: 9182526; 9545220). This complex is composed of two larger subunits (δ and β3A or β3B), a medium subunit (μ3A or μ3B), and a small subunit (σ3A or σ3B). The absence of the β3A subunit (AP3B1) results in the loss of stability of AP3 and leads to degradation of μ3A, to which β3A is directly bound, while the other subunits are variably affected (PMID: 16507770). AP3B1 contains three main domains: the N-terminal head domain, the hinge, and the C-terminal ear domain. It has been reported as a target of IP(7)-mediated pyrophosphorylation (PMID: 19934039). Defects in AP3B1 are the cause of Hermansky-Pudlak syndrome type 2 (HPS2) (PMID: 10024875; 16507770).

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