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验证数据展示

  • WB analysis of Hacat cells using 10618-1-AP (same clone as 10618-1-PBS)

    WB analysis of Hacat cells using 10618-1-AP (same clone as 10618-1-PBS)

    WB result of AP1M2 antibody (10618-1-AP;1:500) with siRNA cell lysate. This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse colon using 10618-1-AP (same clone as 10618-1-PBS)

    WB analysis of mouse colon using 10618-1-AP (same clone as 10618-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 10618-1-AP (AP1M2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 10618-1-AP (same clone as 10618-1-PBS)

    IHC staining of human pancreas cancer using 10618-1-AP (same clone as 10618-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10618-1-AP (AP1M2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 10618-1-AP (same clone as 10618-1-PBS)

    IHC staining of human pancreas cancer using 10618-1-AP (same clone as 10618-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10618-1-AP (AP1M2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

AP1M2 Polyclonal antibody, PBS Only

AP1M2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10618-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

10618-1-PBS targets AP1M2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0961

Product name: Recombinant human AP1M2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-320 aa of BC005021

Sequence: MSASAVFILDVKGKPLISRNYKGDVAMSKIEHFMPLLVQREEEGALAPLLSHGQVHFLWIKHSNLYLVATTSKNANASLVYSFLYKTIEVFCEYFKELEEESIRDNFVIVYELLDELMDFGFPQTTDSKILQEYITQQSNKLETGKSRVPPTVTNAVSWRSEGIKYKKNEVFIDVIESVNLLVNANGSVLLSEIVGTIKLKVFLSGMPELRLGLNDRVLFELTGLSGSKNKSVELEDVKFHQCVRLSRFDNDRTISFIPPDGDFELMSYRLSTQVKPLIWIESVIEKFSHSRVEIMVKAKGQFKKQSVANGVEISVPVPS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adaptor-related protein complex 1, mu 2 subunit
别名
计算分子量 48 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC005021
基因名称 AP1M2
Gene ID (NCBI) 10053
RRIDAB_2242895
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6Q5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AP1M2 is a subunit of the adaptor complex AP-1. It belongs to the adaptor complexes medium subunit family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors.

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