验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HEK-293 cells, mouse testis tissue, PC-3 cells, K-562 cells, Jurkat cells, rat testis tissue |
| Positive IHC detected in | mouse skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HeLa cells, U-251 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83613-6-RR targets AP1M1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, FC (Intra), ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag2757 Product name: Recombinant human AP1M1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 164-423 aa of BC017469 Sequence: IKYRKNEVFLDVIESVNLLVSANGNVLRSEIVGSIKMRVFLSGMPELRLGLNDKVLFDNTGRGKSKSVELEDVKFHQCVRLSRFENDRTISFIPPDGEFELMSYRLNTHVKPLIWIESVIEKHSHSRIEYMIKAKSQFKRRSTANNVEIHIPVPNDADSPKFKTTVGSVKWVPENSEIVWSIKSFPGGKEYLMRAHFGLPSVEAEDKEGKPPISVKFEIPYFTTSGIQVRYLKIIEKSGYQALPWVRYITQNGDYQLRTQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | adaptor-related protein complex 1, mu 1 subunit |
| 别名 | 240553H3, Adaptor protein complex AP-1 subunit mu-1, Adaptor-related protein complex 1 subunit mu-1, AP 1 complex subunit mu 1, AP mu chain family member mu1A |
| 计算分子量 | 423 aa, 49 kDa |
| 观测分子量 | 49 kDa |
| GenBank蛋白编号 | BC017469 |
| 基因名称 | AP1M1 |
| Gene ID (NCBI) | 8907 |
| RRID | AB_3671226 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q9BXS5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AP1M1, also named as CLTNM, Mu-adaptin 1 and Clathrin coat assembly protein AP47, belongs to the adaptor complexes medium subunit family. It is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for AP1M1 antibody 83613-6-RR | Download protocol |
| IHC protocol for AP1M1 antibody 83613-6-RR | Download protocol |
| WB protocol for AP1M1 antibody 83613-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
