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验证数据展示

  • WB analysis of Hacat cells using 12112-1-AP (same clone as 12112-1-PBS)

    WB analysis of Hacat cells using 12112-1-AP (same clone as 12112-1-PBS)

    WB resut of AP1M1 antibody (12112-1-AP, 1:500) with siRNA cell lysate. This data was developed using the same antibody clone with 12112-1-PBS in a different storage buffer formulation.

  • WB analysis of human brain using 12112-1-AP (same clone as 12112-1-PBS)

    WB analysis of human brain using 12112-1-AP (same clone as 12112-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12112-1-AP (AP1M1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12112-1-PBS in a different storage buffer formulation.

  • IP experiment of SH-SY5Y using 12112-1-AP (same clone as 12112-1-PBS)

    IP experiment of SH-SY5Y using 12112-1-AP (same clone as 12112-1-PBS)

    IP result of anti-AP1M1 (IP:12112-1-AP, 4ug; Detection:12112-1-AP 1:500) with SH-SY5Y cells lysate 2500ug. This data was developed using the same antibody clone with 12112-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

AP1M1 Polyclonal antibody, PBS Only

AP1M1 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 12112-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12112-1-PBS targets AP1M1 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2757

Product name: Recombinant human AP1M1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 164-423 aa of BC017469

Sequence: IKYRKNEVFLDVIESVNLLVSANGNVLRSEIVGSIKMRVFLSGMPELRLGLNDKVLFDNTGRGKSKSVELEDVKFHQCVRLSRFENDRTISFIPPDGEFELMSYRLNTHVKPLIWIESVIEKHSHSRIEYMIKAKSQFKRRSTANNVEIHIPVPNDADSPKFKTTVGSVKWVPENSEIVWSIKSFPGGKEYLMRAHFGLPSVEAEDKEGKPPISVKFEIPYFTTSGIQVRYLKIIEKSGYQALPWVRYITQNGDYQLRTQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adaptor-related protein complex 1, mu 1 subunit
别名
计算分子量 423 aa, 49 kDa
观测分子量 49 kDa
GenBank蛋白编号BC017469
基因名称 AP1M1
Gene ID (NCBI) 8907
RRIDAB_2058339
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BXS5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AP1M1, also named as CLTNM, Mu-adaptin 1 and Clathrin coat assembly protein AP47, belongs to the adaptor complexes medium subunit family. It is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

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