验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, Hacat cells |
| Positive IP detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12112-1-AP targets AP1M1 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, canine |
| 免疫原 |
CatNo: Ag2757 Product name: Recombinant human AP1M1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 164-423 aa of BC017469 Sequence: IKYRKNEVFLDVIESVNLLVSANGNVLRSEIVGSIKMRVFLSGMPELRLGLNDKVLFDNTGRGKSKSVELEDVKFHQCVRLSRFENDRTISFIPPDGEFELMSYRLNTHVKPLIWIESVIEKHSHSRIEYMIKAKSQFKRRSTANNVEIHIPVPNDADSPKFKTTVGSVKWVPENSEIVWSIKSFPGGKEYLMRAHFGLPSVEAEDKEGKPPISVKFEIPYFTTSGIQVRYLKIIEKSGYQALPWVRYITQNGDYQLRTQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | adaptor-related protein complex 1, mu 1 subunit |
| 别名 | AP 1 complex subunit mu 1, AP mu chain family member mu1A, AP1M1, AP47, CLAPM2, CLTNM, MU 1A, Mu adaptin 1, Mu1A adaptin |
| 计算分子量 | 423 aa, 49 kDa |
| 观测分子量 | 49 kDa |
| GenBank蛋白编号 | BC017469 |
| 基因名称 | AP1M1 |
| Gene ID (NCBI) | 8907 |
| RRID | AB_2058339 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BXS5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AP1M1, also named as CLTNM, Mu-adaptin 1 and Clathrin coat assembly protein AP47, belongs to the adaptor complexes medium subunit family. It is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for AP1M1 antibody 12112-1-AP | Download protocol |
| WB protocol for AP1M1 antibody 12112-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Plasma membrane contributes to the formation of pre-autophagosomal structures.
| ||
Nat Commun A CRISPR/Cas9 screen reveals proteins at the endosome-Golgi interface that modulate cellular anti-sense oligonucleotide activity. | ||
Nat Commun Reversible control of post-Golgi transport by brefeldin A reveals recycling endosome maturation during glycosylphosphatidylinositol-anchored protein transport. | ||
Cell Commun Signal Crosstalk between KDEL receptor and EGF receptor mediates cell proliferation and migration via STAT3 signaling | ||
Cell Mol Life Sci ELAPOR1 mediated vesicle traffic is required for acrosome biogenesis and male fertility in mice. |
