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验证数据展示

  • WB analysis using 31989-1-AP (same clone as 31989-1-PBS)

    WB analysis using 31989-1-AP (same clone as 31989-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31989-1-AP (ANP32E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31989-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung using 31989-1-AP (same clone as 31989-1-PBS)

    IHC staining of human lung using 31989-1-AP (same clone as 31989-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 31989-1-AP (ANP32E antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31989-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse small intestine using 31989-1-AP (same clone as 31989-1-PBS)

    IHC staining of mouse small intestine using 31989-1-AP (same clone as 31989-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 31989-1-AP (ANP32E antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31989-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 31989-1-AP (same clone as 31989-1-PBS)

    IHC staining of mouse kidney using 31989-1-AP (same clone as 31989-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31989-1-AP (ANP32E antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31989-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse small intestine using 31989-1-AP (same clone as 31989-1-PBS)

    IF Staining of mouse small intestine using 31989-1-AP (same clone as 31989-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using ANP32E antibody (31989-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31989-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse small intestine using 31989-1-AP (same clone as 31989-1-PBS)

    IF Staining of mouse small intestine using 31989-1-AP (same clone as 31989-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using ANP32E antibody (31989-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31989-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 31989-1-AP (same clone as 31989-1-PBS)

    IF Staining of SH-SY5Y using 31989-1-AP (same clone as 31989-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using ANP32E antibody (31989-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 31989-1-PBS in a different storage buffer formulation.

ANP32E Polyclonal antibody, PBS Only

ANP32E Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 31989-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

Acidic (Leucine-Rich) Nuclear Phosphoprotein 32 Family, Member E, Acidic leucine-rich nuclear phosphoprotein 32 family member E, Acidic Nuclear Phosphoprotein 32 Family Member E, LANPL, LANP-L

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31989-1-PBS targets ANP32E in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag33772

Product name: Recombinant human ANP32E protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 159-268 aa of BC003380

Sequence: EEEDDEDGDEDDEEEEENEAGPPEGYEEEEEEEEEEDEDEDEDEDEAGSELGEGEEEVGLSYLMKEEIQDEEDDDDYVEEGEEEEEEEEGGLRGEKRKRDAEDDGEEEDD*

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acidic (leucine-rich) nuclear phosphoprotein 32 family, member E
别名 Acidic (Leucine-Rich) Nuclear Phosphoprotein 32 Family, Member E, Acidic leucine-rich nuclear phosphoprotein 32 family member E, Acidic Nuclear Phosphoprotein 32 Family Member E, LANPL, LANP-L
计算分子量 31 kDa
观测分子量30 kDa
GenBank蛋白编号BC003380
基因名称 ANP32E
Gene ID (NCBI) 81611
RRIDAB_3670167
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9BTT0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Acidic nuclear phosphoprotein 32 family member E (ANP32E) is a histone chaperone that removes H2A.Z from chromatin. ANP32E is implicated in many cellular processes, including chromatin modification and remodeling, gene regulation, protein phosphorylation, and regulation of intracellular trafficking, and contributes to early development and cancer metastasis in mammals. In addition, ANP32E is involved in tumor development and is highly expressed in pancreatic and thyroid cancer cells, and promotes tumorigenesis in gastric cancer (GC) cells by inducing NUF2 expression.

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