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验证数据展示

  • WB analysis using 31989-1-AP

    WB analysis using 31989-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31989-1-AP (ANP32E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung using 31989-1-AP

    IHC staining of human lung using 31989-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 31989-1-AP (ANP32E antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse small intestine using 31989-1-AP

    IHC staining of mouse small intestine using 31989-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 31989-1-AP (ANP32E antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 31989-1-AP

    IHC staining of mouse kidney using 31989-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31989-1-AP (ANP32E antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse small intestine using 31989-1-AP

    IF Staining of mouse small intestine using 31989-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using ANP32E antibody (31989-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse small intestine using 31989-1-AP

    IF Staining of mouse small intestine using 31989-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using ANP32E antibody (31989-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ANP32E Polyclonal antibody

ANP32E Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 31989-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

LANP-L, LANPL, Acidic Nuclear Phosphoprotein 32 Family Member E, Acidic leucine-rich nuclear phosphoprotein 32 family member E, Acidic (Leucine-Rich) Nuclear Phosphoprotein 32 Family, Member E

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, DU 145 cells, HeLa cells, K-562 cells, MCF-7 cells
Positive IHC detected inmouse kidney tissue, human lung tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse small intestine tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31989-1-AP targets ANP32E in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 ANP32E fusion protein Ag33772 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acidic (leucine-rich) nuclear phosphoprotein 32 family, member E
别名 LANP-L, LANPL, Acidic Nuclear Phosphoprotein 32 Family Member E, Acidic leucine-rich nuclear phosphoprotein 32 family member E, Acidic (Leucine-Rich) Nuclear Phosphoprotein 32 Family, Member E
计算分子量 31 kDa
观测分子量30 kDa
GenBank蛋白编号BC003380
基因名称 ANP32E
Gene ID (NCBI) 81611
RRIDAB_3670167
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9BTT0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Acidic nuclear phosphoprotein 32 family member E (ANP32E) is a histone chaperone that removes H2A.Z from chromatin. ANP32E is implicated in many cellular processes, including chromatin modification and remodeling, gene regulation, protein phosphorylation, and regulation of intracellular trafficking, and contributes to early development and cancer metastasis in mammals. In addition, ANP32E is involved in tumor development and is highly expressed in pancreatic and thyroid cancer cells, and promotes tumorigenesis in gastric cancer (GC) cells by inducing NUF2 expression.

实验方案

Product Specific Protocols
WB protocol for ANP32E antibody 31989-1-APDownload protocol
IHC protocol for ANP32E antibody 31989-1-APDownload protocol
IF protocol for ANP32E antibody 31989-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols