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验证数据展示

  • WB analysis of HEK-293 using 24333-1-AP (same clone as 24333-1-PBS)

    WB analysis of HEK-293 using 24333-1-AP (same clone as 24333-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24333-1-AP (ANKRD6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24333-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 24333-1-AP (same clone as 24333-1-PBS)

    IP experiment of HEK-293 using 24333-1-AP (same clone as 24333-1-PBS)

    IP result of anti-ANKRD6 (IP:24333-1-AP, 5ug; Detection:24333-1-AP 1:300) with HEK-293 cells lysate 920ug. This data was developed using the same antibody clone with 24333-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse ovary using 24333-1-AP (same clone as 24333-1-PBS)

    IHC staining of mouse ovary using 24333-1-AP (same clone as 24333-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 24333-1-AP (ANKRD6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24333-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse ovary using 24333-1-AP (same clone as 24333-1-PBS)

    IHC staining of mouse ovary using 24333-1-AP (same clone as 24333-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 24333-1-AP (ANKRD6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24333-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ANKRD6 Polyclonal antibody, PBS Only

ANKRD6 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 24333-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, Indirect ELISA

别名

Diversin, Ankyrin repeat domain-containing protein 6, ANKRD 6

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

24333-1-PBS targets ANKRD6 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19328

Product name: Recombinant human ANKRD6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-260 aa of BC042173

Sequence: MSQQDAVAALSERLLVAAYKGQTENVVQLINKGARVAVTKHGRTPLHLAANKGHLPVVQILLKAGCDLDVQDDGDQTALHRATVVGNTEIIAALIHEGCALDRQDKDGNTALHEASWHGFSQSAKLLVKAGANVLAKNKAGNTALHLACQNSHSQSTRVLLLAGSRADLKNNAGDTCLHVAARYNHLSIIRLLLTAFCSVHEKNQAGDTALHVAAALNHKKVAKILLEAGADTTIVNNAGQTPLETARYHNNPEVALLLT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ankyrin repeat domain 6
别名 Diversin, Ankyrin repeat domain-containing protein 6, ANKRD 6
计算分子量 727 aa, 80 kDa
观测分子量 80-90 kDa
GenBank蛋白编号BC042173
基因名称 ANKRD6
Gene ID (NCBI) 22881
RRIDAB_2879498
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2G4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ankrd6 (Ankyrin repeat domain 6) is a distant homolog of the fly PCP mediator diego and acts as a molecular switch between both Wnt pathways. It promotes signaling of the non canonical Wnt/PCP pathway and simultaneously inhibits gene transcription through the canonical Wnt/β-catenin pathway.

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