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  • Featured Product
  • KD/KO Validated

ANKRD6 Polyclonal antibody

ANKRD6 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 24333-1-AP

Print datasheet

Synonyms

ANKRD6, ankyrin repeat domain 6, DIVERSIN, KIAA0957

验证数据展示

  • WB analysis of HEK-293 using 24333-1-AP

    WB analysis of HEK-293 using 24333-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24333-1-AP (ANKRD6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 24333-1-AP

    IP experiment of HEK-293 using 24333-1-AP

    IP result of anti-ANKRD6 (IP:24333-1-AP, 5ug; Detection:24333-1-AP 1:300) with HEK-293 cells lysate 920ug.

  • IHC staining of human kidney using 24333-1-AP

    IHC staining of human kidney using 24333-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24333-1-AP (ANKRD6 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human ovary using 24333-1-AP

    IHC staining of human ovary using 24333-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 24333-1-AP (ANKRD6 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHEK-293 cells, fetal human brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman ovary tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24333-1-AP targets ANKRD6 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen ANKRD6 fusion protein Ag19328 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ankyrin repeat domain 6
Synonyms ANKRD6, ankyrin repeat domain 6, DIVERSIN, KIAA0957
Calculated Molecular Weight 727 aa, 80 kDa
Observed Molecular Weight 80-90 kDa
GenBank Accession NumberBC042173
Gene Symbol ANKRD6
Gene ID (NCBI) 22881
RRIDAB_2879498
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2G4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ankrd6 (Ankyrin repeat domain 6) is a distant homolog of the fly PCP mediator diego and acts as a molecular switch between both Wnt pathways. It promotes signaling of the non canonical Wnt/PCP pathway and simultaneously inhibits gene transcription through the canonical Wnt/β-catenin pathway.

实验方案

Product Specific Protocols
WB protocol for ANKRD6 antibody 24333-1-APDownload protocol
IHC protocol for ANKRD6 antibody 24333-1-APDownload protocol
IP protocol for ANKRD6 antibody 24333-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Chin J Cancer Res

Pan-cancer analyses demonstrate that ANKRD6 is associated with a poor prognosis and correlates with M2 macrophage infiltration in colon cancer.

Authors - Rui Bai
View Article
humanWB

Mol Carcinog

Role of miR-214 in regulation of β-catenin and the malignant phenotype of melanoma.

Authors - Kirthana Prabhakar
  • KD Validated
View Article