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验证数据展示

  • WB analysis of mouse testis using 25738-1-AP

    WB analysis of mouse testis using 25738-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25738-1-AP (ANKRD54 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 25738-1-AP

    IHC staining of mouse testis using 25738-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25738-1-AP (ANKRD54 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 25738-1-AP

    IHC staining of mouse testis using 25738-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25738-1-AP (ANKRD54 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ANKRD54 Polyclonal antibody

ANKRD54 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25738-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Lyn-interacting ankyrin repeat protein, LIAR, Ankyrin repeat domain-containing protein 54, ANKRD 54

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, K-562 cells, PC-3 cells
Positive IHC detected inmouse testis tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25738-1-AP targets ANKRD54 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 ANKRD54 fusion protein Ag22759 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ankyrin repeat domain 54
别名 Lyn-interacting ankyrin repeat protein, LIAR, Ankyrin repeat domain-containing protein 54, ANKRD 54
计算分子量 300 aa, 33 kDa
观测分子量 33-35 kDa
GenBank蛋白编号BC066909
基因名称 ANKRD54
Gene ID (NCBI) 129138
RRIDAB_2880218
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6NXT1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ANKRD54 antibody 25738-1-APDownload protocol
IHC protocol for ANKRD54 antibody 25738-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols