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ANKRD50 Polyclonal antibody

ANKRD50 Polyclonal Antibody for IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 24032-1-AP

Print datasheet

Synonyms

ANKRD50, ankyrin repeat domain 50, KIAA1223

验证数据展示

  • WB analysis of HEK-293 using 24032-1-AP

    WB analysis of HEK-293 using 24032-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24032-1-AP (ANKRD50 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 24032-1-AP

    IP experiment of HEK-293 using 24032-1-AP

    IP result of anti-ANKRD50 (IP:24032-1-AP, 5ug; Detection:24032-1-AP 1:300) with HEK-293 cells lysate 4000ug.

  • IF Staining of HEK-293 using 24032-1-AP

    IF Staining of HEK-293 using 24032-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 24032-1-AP (ANKRD50 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IF detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24032-1-AP is ANKRD50 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IP, WB, ELISA
Tested Reactivity human
Immunogen ANKRD50 fusion protein Ag21237 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ankyrin repeat domain 50
Synonyms ANKRD50, ankyrin repeat domain 50, KIAA1223
Calculated Molecular Weight 1429 aa, 156 kDa
Observed Molecular Weight 137-156 kDa
GenBank Accession NumberBC024725
Gene Symbol ANKRD50
Gene ID (NCBI) 57182
RRIDAB_2879411
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9ULJ7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ANKRD50 antibody 24032-1-APDownload protocol
IF protocol for ANKRD50 antibody 24032-1-APDownload protocol
IP protocol for ANKRD50 antibody 24032-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols