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验证数据展示

  • WB analysis of HEK-293 using 24032-1-AP

    WB analysis of HEK-293 using 24032-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24032-1-AP (ANKRD50 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 24032-1-AP

    IP experiment of HEK-293 using 24032-1-AP

    IP result of anti-ANKRD50 (IP:24032-1-AP, 5ug; Detection:24032-1-AP 1:300) with HEK-293 cells lysate 4000ug.

  • IF Staining of HEK-293 using 24032-1-AP

    IF Staining of HEK-293 using 24032-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 24032-1-AP (ANKRD50 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

ANKRD50 Polyclonal antibody

ANKRD50 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 24032-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

ANKRD50, ankyrin repeat domain 50, KIAA1223

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24032-1-AP targets ANKRD50 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 ANKRD50 fusion protein Ag21237 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ankyrin repeat domain 50
别名 ANKRD50, ankyrin repeat domain 50, KIAA1223
计算分子量 1429 aa, 156 kDa
观测分子量 137-156 kDa
GenBank蛋白编号BC024725
基因名称 ANKRD50
Gene ID (NCBI) 57182
RRIDAB_2879411
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9ULJ7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ANKRD50 antibody 24032-1-APDownload protocol
IF protocol for ANKRD50 antibody 24032-1-APDownload protocol
IP protocol for ANKRD50 antibody 24032-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols