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  • Featured Product
  • KD/KO Validated

APC2 Polyclonal antibody

APC2 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 13559-1-AP

Print datasheet

Synonyms

ANAPC2, APC2, Cyclosome subunit 2, KIAA1406, RP11 350O14.5

验证数据展示

  • IHC staining of human breast cancer using 13559-1-AP

    IHC staining of human breast cancer using 13559-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13559-1-AP (APC2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APC2 antibody included
  • IHC staining of human breast cancer using 13559-1-AP

    IHC staining of human breast cancer using 13559-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13559-1-AP (APC2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APC2 antibody included
  • WB analysis using 13559-1-AP

    WB analysis using 13559-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13559-1-AP (APC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 13559-1-AP

    IF Staining of MCF-7 using 13559-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 13559-1-AP (APC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of MCF-7 using 13559-1-AP

    IP experiment of MCF-7 using 13559-1-AP

    IP result of anti-APC2 (IP:13559-1-AP, 4ug; Detection:13559-1-AP 1:300) with MCF-7 cells lysate 3200ug.

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy APC2 Ready-To-Use IHC Kit. APC2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13559-1-AP targets APC2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen APC2 fusion protein Ag4427 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name anaphase promoting complex subunit 2
Synonyms ANAPC2, APC2, Cyclosome subunit 2, KIAA1406, RP11 350O14.5
Calculated Molecular Weight 822 aa, 94 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC032503
Gene Symbol ANAPC2
Gene ID (NCBI) 29882
RRIDAB_2227055
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJX6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for APC2 antibody 13559-1-APDownload protocol
IHC protocol for APC2 antibody 13559-1-APDownload protocol
IF protocol for APC2 antibody 13559-1-APDownload protocol
IP protocol for APC2 antibody 13559-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF

FASEB J

APC2CDH1 negatively regulates agrin signaling by promoting the ubiquitination and proteolytic degradation of DOK7.

Authors - Aizhong Chen
  • KD Validated
View Article
humanWB

FEBS J

The MAD2B-APC/C-MDM2 axis mediates acute kidney injury by modulating p53

Authors - Zhe Hao
View Article
mouseIF

Nat Commun

CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I

Authors - Cheng-Jie Zhou
View Article