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验证数据展示

  • IF Staining of rat brain using CL488-80209-5

    IF Staining of rat brain using CL488-80209-5

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using CoraLite® Plus 488 AMPK Alpha antibody (CL488-80209-5, Clone: 4N5 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CoraLite® Plus 488-conjugated AMPK Alpha Recombinant monoclonal antibody

AMPK Alpha Uni-rAb® Recombinant Antibody for IF-P
Cat No. CL488-80209-5

产品说明书

CloneNo. 4N5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF-P

别名

AMPK Alpha 1, PRKAA1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, AAPK1, ACACA kinase

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF-P detected inrat brain tissue
Planning an IHC experiment? We recommend our IHCeasy PRKAA1 Ready-To-Use IHC Kit. PRKAA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80209-5-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-80209-5 targets AMPK Alpha in IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1367

Product name: Recombinant human AMPK alpha protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-207 aa of BC012622

Sequence: MATAEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRDCNIIRILTSQFTNYQH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein kinase, AMP-activated, alpha 1 catalytic subunit
别名 AMPK Alpha 1, PRKAA1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, AAPK1, ACACA kinase
计算分子量 63 kDa
观测分子量 64 kDa
GenBank蛋白编号BC012622
基因名称 AMPK Alpha 1
Gene ID (NCBI) 5562
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13131
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing. 80209-5-RR can recognize both AMPK Alpha 1 and AMPK Alpha 2.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 AMPK Alpha antibody CL488-80209-5Download protocol
Standard Protocols
Click here to view our Standard Protocols
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