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验证数据展示

  • WB analysis of human liver using 15710-1-AP

    WB analysis of human liver using 15710-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15710-1-AP (AMPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human cerebellum using 15710-1-AP

    IHC staining of human cerebellum using 15710-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 15710-1-AP (AMPD2 Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with AMPD2 antibody included
  • IHC staining of human cerebellum using 15710-1-AP

    IHC staining of human cerebellum using 15710-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 15710-1-AP (AMPD2 Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with AMPD2 antibody included

AMPD2 Polyclonal antibody

AMPD2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15710-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

AMP deaminase 2, AMP deaminase isoform L, AMPD2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman liver tissue
Positive IHC detected inhuman cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy AMPD2 Ready-To-Use IHC Kit. AMPD2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

15710-1-AP targets AMPD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 AMPD2 fusion protein Ag8335 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adenosine monophosphate deaminase 2 (isoform L)
别名 AMP deaminase 2, AMP deaminase isoform L, AMPD2
计算分子量879aa,101 kDa; 798aa,92 kDa
观测分子量 101 kDa
GenBank蛋白编号BC007711
基因名称 AMPD2
Gene ID (NCBI) 271
RRIDAB_2242657
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ01433
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Adenosine monophosphate deaminase (AMPD) catalyzes the deamination of AMP to IMP and plays an important role in the purine nucleotide cycle. Three AMPDs are present in mammals named AMPD1/2/3. AMPD2 is the main activity present in adult human liver and is widely expressed in non-muscle tissues and cells (PMID: 8526848). AMPD2 has some isoforms with the molecular mass of 88-101 kDa.

实验方案

Product Specific Protocols
WB protocol for AMPD2 antibody 15710-1-APDownload protocol
IHC protocol for AMPD2 antibody 15710-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

World J Clin Cases

Overexpression of AMPD2 indicates poor prognosis in colorectal cancer patients via the Notch3 signaling pathway.

Authors - Qi-Zhong Gao
View Article