验证数据展示
经过测试的应用
| Positive WB detected in | L02 cells |
Planning an IHC experiment? We recommend our IHCeasy ALPL Ready-To-Use IHC Kit. ALPL primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82915-2-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-82915-2 targets ALPL in WB applications and shows reactivity with human samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag1664 Product name: Recombinant human ALPL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 23-330 aa of BC021289 Sequence: EKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | alkaline phosphatase, liver/bone/kidney |
| 别名 | Alkaline phosphatase, Alkaline phosphatase, tissue-nonspecific isozyme, Alkaline phosphatase tissue nonspecific isozyme, Alkaline phosphatase liver/bone/kidney isozyme, AKP2 |
| 计算分子量 | 57 kDa |
| 观测分子量 | 80 kDa |
| GenBank蛋白编号 | BC021289 |
| 基因名称 | ALPL |
| Gene ID (NCBI) | 249 |
| RRID | AB_3673746 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P05186 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 ALPL antibody CL750-82915-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
