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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68719-1-Ig (same clone as 68719-1-PBS)

    WB analysis using 68719-1-Ig (same clone as 68719-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68719-1-Ig (ALPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68719-1-PBS in a different storage buffer formulation.

  • IHC staining of rat adrenal gland using 68719-1-Ig (same clone as 68719-1-PBS)

    IHC staining of rat adrenal gland using 68719-1-Ig (same clone as 68719-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 68719-1-Ig (ALPL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68719-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALPL antibody included

ALPL Monoclonal antibody, PBS Only

ALPL Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 68719-1-PBS

产品说明书

CloneNo. 2E3C1

宿主/亚型

Mouse / IgG2a

种属反应性

human, pig, rabbit

应用

WB, IHC, Indirect ELISA

别名

Alkaline phosphatase, 2E3C1, AKP2, Alkaline phosphatase liver/bone/kidney isozyme, Alkaline phosphatase tissue nonspecific isozyme

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ALPL Ready-To-Use IHC Kit. ALPL primary antibody included.

产品信息

68719-1-PBS targets ALPL in WB, IHC, Indirect ELISA applications and shows reactivity with human, pig, rabbit samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, pig, rabbit
免疫原

CatNo: Ag33678

Product name: Recombinant human ALPL protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 23-330 aa of BC021289

Sequence: EKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLL

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 alkaline phosphatase, liver/bone/kidney
别名 Alkaline phosphatase, 2E3C1, AKP2, Alkaline phosphatase liver/bone/kidney isozyme, Alkaline phosphatase tissue nonspecific isozyme
计算分子量 57 kDa
观测分子量 80 kDa
GenBank蛋白编号BC021289
基因名称 ALPL
Gene ID (NCBI) 249
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP05186
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa(PMID:18724009). ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H (Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol (GPI)(PMID:10839996).