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验证数据展示

  • WB analysis using 68719-1-Ig

    WB analysis using 68719-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68719-1-Ig (ALPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat adrenal gland using 68719-1-Ig

    IHC staining of rat adrenal gland using 68719-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 68719-1-Ig (ALPL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALPL antibody included

ALPL Monoclonal antibody

ALPL Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68719-1-Ig

产品说明书

CloneNo. 2E3C1

宿主/亚型

Mouse / IgG2a

种属反应性

human, pig, rabbit

应用

WB, IHC, ELISA

别名

Alkaline phosphatase, 2E3C1, AKP2, Alkaline phosphatase liver/bone/kidney isozyme, Alkaline phosphatase tissue nonspecific isozyme

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inpig kidney tissue, rabbit kidney tissue
Positive IHC detected inrat adrenal gland tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ALPL Ready-To-Use IHC Kit. ALPL primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68719-1-Ig targets ALPL in WB, IHC, ELISA applications and shows reactivity with human, pig, rabbit samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, pig, rabbit
免疫原 ALPL fusion protein Ag33678 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 alkaline phosphatase, liver/bone/kidney
别名 Alkaline phosphatase, 2E3C1, AKP2, Alkaline phosphatase liver/bone/kidney isozyme, Alkaline phosphatase tissue nonspecific isozyme
计算分子量 57 kDa
观测分子量 80 kDa
GenBank蛋白编号BC021289
基因名称 ALPL
Gene ID (NCBI) 249
RRIDAB_3670412
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP05186
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa(PMID:18724009). ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H (Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol (GPI)(PMID:10839996).

实验方案

Product Specific Protocols
WB protocol for ALPL antibody 68719-1-IgDownload protocol
IHC protocol for ALPL antibody 68719-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols