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验证数据展示

  • WB analysis of HSC-T6 using 67811-1-Ig (same clone as 67811-1-PBS)

    WB analysis of HSC-T6 using 67811-1-Ig (same clone as 67811-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 67811-1-Ig (same clone as 67811-1-PBS)

    WB analysis of NIH/3T3 using 67811-1-Ig (same clone as 67811-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

  • WB analysis of 4T1 using 67811-1-Ig (same clone as 67811-1-PBS)

    WB analysis of 4T1 using 67811-1-Ig (same clone as 67811-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67811-1-Ig (same clone as 67811-1-PBS)

    WB analysis of HeLa using 67811-1-Ig (same clone as 67811-1-PBS)

    WB result of ALKBH5 antibody (67811-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALKBH5 transfected HeLa cells. This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 67811-1-Ig (same clone as 67811-1-PBS)

    IHC staining of mouse testis using 67811-1-Ig (same clone as 67811-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67811-1-Ig (ALKBH5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALKBH5 antibody included
  • IHC staining of mouse testis using 67811-1-Ig (same clone as 67811-1-PBS)

    IHC staining of mouse testis using 67811-1-Ig (same clone as 67811-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67811-1-Ig (ALKBH5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67811-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALKBH5 antibody included
  • Featured Product
  • KD/KO验证

ALKBH5 Monoclonal antibody, PBS Only

ALKBH5 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67811-1-PBS

产品说明书

CloneNo. 2D2B2

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat

应用

WB, IHC, Indirect ELISA

别名

ABH5, ALKBH5, FLJ20308, OFOXD, OFOXD1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ALKBH5 Ready-To-Use IHC Kit. ALKBH5 primary antibody included.

产品信息

67811-1-PBS targets ALKBH5 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原

CatNo: Ag10490

Product name: Recombinant human ALKBH5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 57-383 aa of BC062339

Sequence: KRKYQEDSDPERSDYEEQQLQKEEEARKVKSGIRQMRLFSQDECAKIEARIDEVVSRAEKGLYNEHTVDRAPLRNKYFFGEGYTYGAQLQKRGPGQERLYPPGDVDEIPEWVHQLVIQKLVEHRVIPEGFVNSAVINDYQPGGCIVSHVDPIHIFERPIVSVSFFSDSALCFGCKFQFKPIRVSEPVLSLPVRRGSVTVLSGYAADEITHCIRPQDIKERRAVIILRKTRLDAPRLETKSLSSSVLPPSYASDRLSGNNRDPALKPKRSHRKADPDAAHRPRILEMDKEENRRSVLLPTHRRRGSFSSENYWRKSYESSEDCSEAAG

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 alkB, alkylation repair homolog 5 (E. coli)
别名 ABH5, ALKBH5, FLJ20308, OFOXD, OFOXD1
计算分子量 458 aa, 51 kDa
观测分子量40-44 kDa
GenBank蛋白编号BC062339
基因名称 ALKBH5
Gene ID (NCBI) 54890
RRIDAB_2918574
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ6P6C2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.