验证数据展示
经过测试的应用
| Positive WB detected in | 4T1 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IHC | See 2 publications below |
产品信息
67811-1-Ig targets ALKBH5 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | Human, Mouse, Rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag10490 Product name: Recombinant human ALKBH5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 57-383 aa of BC062339 Sequence: KRKYQEDSDPERSDYEEQQLQKEEEARKVKSGIRQMRLFSQDECAKIEARIDEVVSRAEKGLYNEHTVDRAPLRNKYFFGEGYTYGAQLQKRGPGQERLYPPGDVDEIPEWVHQLVIQKLVEHRVIPEGFVNSAVINDYQPGGCIVSHVDPIHIFERPIVSVSFFSDSALCFGCKFQFKPIRVSEPVLSLPVRRGSVTVLSGYAADEITHCIRPQDIKERRAVIILRKTRLDAPRLETKSLSSSVLPPSYASDRLSGNNRDPALKPKRSHRKADPDAAHRPRILEMDKEENRRSVLLPTHRRRGSFSSENYWRKSYESSEDCSEAAG 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | alkB, alkylation repair homolog 5 (E. coli) |
| 别名 | ABH5, ALKBH5, FLJ20308, OFOXD, OFOXD1 |
| 计算分子量 | 458 aa, 51 kDa |
| 观测分子量 | 40-44 kDa |
| GenBank蛋白编号 | BC062339 |
| 基因名称 | ALKBH5 |
| Gene ID (NCBI) | 54890 |
| RRID | AB_2918574 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q6P6C2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ALKBH5 antibody 67811-1-Ig | Download protocol |
| WB protocol for ALKBH5 antibody 67811-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Apoptosis NRP1 contributes to stemness and potentiates radioresistance via WTAP-mediated m6A methylation of Bcl-2 mRNA in breast cancer | ||
Cell Signal FTO alleviates cerebral ischemia/reperfusion-induced neuroinflammation by decreasing cGAS mRNA stability in an m6A-dependent manner | ||
Acta Physiol (Oxf) Landscape of transcriptome-wide m6A modification in diabetic liver reveals rewiring of PI3K-Akt signaling after physical exercise | ||
Science Liver ALKBH5 regulates glucose and lipid homeostasis independently through GCGR and mTORC1 signaling
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